大豆多糖对环磷酰胺抗肿瘤作用的减毒增效机制研究  

Mechanism of attenuation and potentiation of the soybean polysaccharide to antitumor of cyclophosphamide

作　　者：张秀娟[1,2] 栾宗泽 孙向菊[2] 严松[3] 李其成[2] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心生物安全评价研究所国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076 [2]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [3]黑龙江省农业科学院食品加工研究所,黑龙江哈尔滨150086

出　　处：《食品工业科技》2014年第19期359-361,383,共4页Science and Technology of Food Industry

摘　　要：研究大豆多糖能否减轻环磷酰胺对机体免疫系统和造血系统的影响。以昆明种小鼠为研究对象,腋下接种S180肉瘤,腹腔注射环磷酰胺25mg/kg·d以及大豆多糖50、100、200mg/kg·d。测定S180荷瘤小鼠外周血中白细胞数量,ELISA法检测细胞因子TNF-α的变化,流式细胞仪检测S180荷瘤小鼠T淋巴细胞亚群CD+4/CD+8变化。结果显示:与单独应用环磷酰胺相比,大豆多糖可以提高荷瘤小鼠的外周血中白细胞数量;提高荷瘤小鼠血清中细胞因子TNF-α水平。随大豆多糖给药剂量的升高,CD+4细胞比例变化不明显,CD+8细胞比例降低,CD+4/CD+8升高。故大豆多糖能够通过调节荷瘤小鼠免疫功能而增强环磷酰胺的抗肿瘤作用,并减轻其毒副作用。To study the influences of soybean polysaccharides combined with cyclophosphamide on immune system and hematopoietic system.This experiment with axillary inoculated S180 sarcoma of KM mice as the carrier, intraperitoneal injection of cyclophosphamide 25mg/kg.d and soybean polysaccharides 50, 100 and 200mg/kg. d respectively.The amount of peripheral blood leukocyte of mice were counted ;TNF-α was determined by ELISA and splenic lymphocyte surface value of CD4＋/CD8＋ was detected by flow cytometry.The results showed that groups treated with soybean polysaccharides could improve the leukocyte in peripheral blood of tumor-burdened mice and improve the level of cytokine TNF-α in peripheral blood compared with cyclophosphamide model group.The results showed that the quantity of CD4＋ cells changed inconspicuously with the increasing of soybean polysaccharides, the quantity of CD8＋ cells decreased and the value of CD4＋/CD8＋ rised. So soybean polysaccharides could enhance anti-tumor effect of cyclophosphamide, strengthen the immune function and lighten toxicity of tumor-burdened mice.

关 键 词：大豆多糖 环磷酰胺 减毒增效作用 免疫增强作用 CD4+ CD8+ 

分 类 号：TS201.4[轻工技术与工程—食品科学]