DA201-C大孔吸附树脂纯化大豆多肽条件优化  

Optimization of Conditions for Purifying Soybean Peptides with DA201-C Macroporous Adsorptive Resins

作　　者：马寒冰[1] 廖永红[1] 徐曼[1] 张弦[1] 

机构地区：[1]北京工商大学食品学院,北京市食品风味化学重点实验室,食品添加剂与配料北京高校工程研究中心,北京100048

出　　处：《中国调味品》2014年第9期43-48,共6页China Condiment

基　　金：国家"十二五"科技支撑计划项目(2011BAD23B01);<1万吨/年富含蛋白质发酵残渣高值化利用技术及示范>(2011BAC11B06);<我国传统发酵食品制造安全预警技术研究与应用>(2012BAK17B11);北京工商大学研究生科研学术创新基金项目

摘　　要：采用大孔吸附树脂纯化豆粕蛋白酶解液中的大豆多肽,经静态吸附和解吸试验表明:DA201-C大孔吸附树脂对大豆多肽的吸附性能及脱盐效果优于其他8种树脂,其最佳工艺条件:上样流速1.08mL/min、上样浓度13.6mg/mL、上样体积240mL、水洗脱体积400mL、75%乙醇洗脱体积500mL,在此条件下,大豆多肽含量提高了70.35%、糖含量降低了70.8%、盐含量降低了93.93%、大豆多肽回收率为62.16%。Purify soybean peptides with macroporous adsorptive resins, and the results of static adsorption and desorption show that DA201-C macroporous adsorption resin has higher adsorption and desalination capability to soybean peptides than other 8 resins. The optimum conditions are as follows：flow rate of sampling is 1.08 mL/min,substrate concentration of sampling is 13.6 mg/mL, volume of sampling is 240 mL,elution volume of water is 400 mL,and elution volume of 75% ethanol is 500 mL.Under these conditions,content of soybean peptides increased by 70.35%,content of sugar decreased by 70.8%,content of salt decreased by 93.93% and the recovery of soybean peptides is 62.16%.

关 键 词：大豆多肽 高品质酱油 大孔吸附树脂 纯化 

分 类 号：TS201.25[轻工技术与工程—食品科学]