碱性蛋白酶酶解提取羊血血红素工艺条件优化  

Process optimization of the alkaline enzymatic extracting heme from sheep blood

作　　者：祝霞[1] 李颍[1] 付文力[2] 杜娜[1] 习京 韩舜愈[1] 盛文军[1] 牛黎莉[1] 杨学山[2] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《食品工业科技》2014年第18期199-202,共4页Science and Technology of Food Industry

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2010-03);甘肃农业大学大学生科研训练计划项目(20130815)

摘　　要：以羊血为试材,血红素得率为指标,在加酶量、酶解pH、酶解温度和酶解时间等单因素实验基础上,采用正交实验优化工艺参数。结果表明最佳反应参数为碱性蛋白酶加酶量8000U/g血红蛋白、酶解pH8.5、酶解温度55℃、酶解时间4h,在此条件血红素得率达到85.3%,即1L羊血可提取3.93g血红素。该法所得产品的安全性高,适合工业化生产。Sheep blood as the research material and the yield of heme was measure indicate. Orthogonal array design generated based on single-factor experiments was employed to optimize conditions. The extraction factors such as the amount of enzyme,pH,react temperature and react time were investigated. The results showed that the optimal amount of enzyme was 8000U/g,pH8.5,react temperature was 55℃ ,react time was 4h,under these optimal conditions,the yield of heme was 85.3% ,that was equivalent to 3.93g heme of 1L sheep blood. This method had higher safety than others, it was suitable for industrialized production.

关 键 词：碱性蛋白酶 羊血 血红素 工艺条件 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]