液-质联机分析牛脂甘油酯组成及结构  

Analysis of Structure and Composition of Glycerides of Tallow by High Performance Liquid Chromatography and Mass Spectrometry

作　　者：曾慧英[1] 公敬欣[1] 杨峥[1] 张玲[1] 都荣强[1] 谢建春[1] 孙宝国[1] 

机构地区：[1]北京市食品风味化学重点实验室北京市高等学校食品添加剂与配料工程中心北京工商大学,北京100048

出　　处：《中国食品学报》2014年第9期247-256,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(No.31171755,31371838);北京市自然科学基金项目(No.21220213);北京市自然科学基金联合资助项目(KZ201010011011);北京市教委科技发展计划重点

摘　　要：采用皂化/甲酯化GC-MS分析牛脂的脂肪酸组成,主要为棕榈酸(39.86%)、油酸(37.58%)、硬脂酸(11.14%)。采用反相高效液相色谱(RP-HPLC)-大气压化学电离质谱(APCI-MS)及电喷雾电离质谱(ESI-MS)分析牛脂的甘油酯组成,根据ESI准分子离子,APCI准分子离子、二酰基甘油碎片离子、离子阱碰撞诱导解离形成的二级质谱,以及甘油酯在反相色谱上出峰的等价碳原子数原则,并结合脂肪酸组成分析,鉴定出26种甘油酯,包括7种甘二酯和19种甘三酯,其中含量较高的为1-棕榈酸-2-油酸-3-亚油酸甘三酯(11.74%)、1-油酸-2,3-二亚油酸甘三酯(10.97%)、1,3-二油酸-2-亚油酸甘三酯(10.25%)。First the fatty acid composition of tallow was analyzed by GC-MS after saponification and transmethyla- tion. It turned out that palmitie acid （39.86%）, oleic acid （37.58%）, and stearic acid （11.14%） were the majors. Then reverse phase high performance liquid chromatography （RP-HPLC） coupled with atmospheric pressure chemical ionization mass spectrometry （APCI-MS） as well as electrospray ionization mass spectrometry （ESI-MS） was carried out to analyze glyeerides in the tallow. Based on quasi-molecular ion peaks in both APCI and ESI-MS, diacylglycerol fragment ions in APCI-MS, fragment ions in APCI-MS/MS by ion trap collision-induced dissociation, and the equivalence-carbon-number （ECN） rule in elution of glyeerides by RP-HPLC as well as the fatty acid composition that had been known, twenty-six glycerides including seven diacylglycerols and nineteen triacylglycerols were identified, among which 1-palmitoyl-2- oleoyl-3-1inoleoyl-glycerol （11.74%）, 1-oleoyl-2,3-di-linoleoyl-glycerol （10.97% ） and 1,3-di-oleoyl 2-1inoleoyl-glycerol （10.25%） dominated.

关 键 词：牛脂 脂肪酸 甘三酯 反相高效液相色谱 大气压化学电离质谱 电喷雾电离质谱 

分 类 号：O657.63[理学—分析化学] TS227[理学—化学]