牛血清白蛋白-刺槐豆胶-壳聚糖缓释纳米微球的制备、表征及其体外释放性能研究  

Preparation,characterization and in vitro releasing property of bovine serum albumin-locust bean gum-chitosan sustained-release microspheres

作　　者：高义霞[1] 周向军[1] 王廷璞[1] 袁毅君[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001

出　　处：《食品工业科技》2014年第20期332-335,348,共5页Science and Technology of Food Industry

基　　金：天水师范学院"青蓝"人才工程基金资助;国家自然基金(81360619)

摘　　要：以刺槐豆胶和壳聚糖为载体,戊二醛为交联剂,牛血清白蛋白(BSA)为模型药物,采用交联法制备BSA-刺槐豆胶-壳聚糖缓释纳米微球。通过单因素实验探讨不同戊二醛浓度、载体与BSA质量比、水相:油相体积比及交联时间对纳米微球制备的影响,对纳米微球进行红外光谱、热重、元素分析、动态光散射及形貌等表征,并测定体外释放性能。结果表明,纳米微球最佳制备工艺为:戊二醛浓度1.25%,载体与BSA比为20∶1,水相与油相比6∶1,交联时间60min,在此条件下纳米微球包封率为62.32%±0.39%,载药量3.45%±1.43%。形貌分析表明,纳米微球尺寸分布均一,平均粒径约为80nm。纳米微球具有一定的缓释能力,在模拟小肠中释放最快,胃中次之,大肠中最慢。Bovine serum albumin（BSA）-Iocust bean gum-chitosan sustained-release microspheres were prepared by cross-linking method using locust bean gum and chitosan as carrier,glutaraldehyde cross-linking agent and BSA model drug. The effects of different glutaraldehyde concentration,the ratio of carrier to BSA and water to oil phase,and cross-linking time on microspheres preparation were investigated by single factor experiments. The resulting microspheres were characterized by infrared spectrum,thermogravimetric analysis,element analysis,dynamic light scattering and morphology analysis. At the same time, its releasing property was also determined. The results showed that the optimal preparation technology for microspheres were glutaraldehyde concentration 1.25% ,the ratio of carrier to BSA and water to oil phase 20：1 and 6：1 ,respectively and crosslinking time 60min. Under these conditions,encapsulation efficiency and drug loading for microspheres were 62.32%±0.39% and 3.45%±1.43%,respectively. The morphology of microspheres was in uniform size with the average diameter of 80nm. The microspheres had certain sustained-release capability,and their releasing rate was as follows ：small intestine〉stomach〉large intestine.

关 键 词：刺槐豆胶 壳聚糖 牛血清白蛋白 缓释 

分 类 号：TS201.1[轻工技术与工程—食品科学]