武夷名丛茶多糖清除羟基自由基活性研究  

Study on Antioxidant Activity of Wuyi Ming Cong Tea Polysaccharides

作　　者：石玉涛[1] 郑超[1,2] 黄华娟[1] 吴玲凤[1] 谢燕云 严玉琢 郑淑琳 罗盛财 

机构地区：[1]武夷学院茶与食品学院/福建省武夷茶资源创新利用重点实验室/福建省高校茶叶工程研究中心/中国乌龙茶产业协同创新中心(培育),福建武夷山354300 [2]青岛农业大学茶叶研究所,山东青岛266109 [3]武夷山龟岩茶业有限公司,福建武夷山354300

出　　处：《中国农学通报》2014年第25期184-188,共5页Chinese Agricultural Science Bulletin

基　　金：福建省教育厅A类科技项目"高多糖活性武夷岩茶种质资源筛选及茶多糖构效关系研究"(JA11260);武夷学院青年教师专项科研基金项目"高多糖活性武夷岩茶种质资源筛选与利用"(xq201014);武夷学院大学生创新性试验计划项目"高多糖活性武夷岩茶种质筛选与武夷岩茶多糖保健含片研制"(xj2011014)

摘　　要：以‘金毛猴’、‘金罗汉’、‘留兰香’、‘玉麒麟’、‘玉观音’等5份武夷名丛种质资源为研究对象,测定了主要生化成分含量,通过水提醇沉法提取茶多糖,分析了茶多糖的基本组分,并采用D-脱氧核糖-铁体系法研究了茶多糖清除羟基自由基的活性。结果表明:5份名丛中游离氨基酸、茶多酚含量存在极显著差异,可溶性糖含量存在显著差异,茶多糖中糖醛酸、中性糖的含量存在极显著差异,表明武夷名丛种质资源具有生化成分多样性;‘玉观音’、‘金毛猴’、‘玉麒麟’、‘金罗汉’、‘留兰香’茶多糖对羟基自由基的清除活性存在显著差异,其IC50分别为4.37、4.80、6.21、6.31、6.75 mg/mL,均高于阳性对照Vc(IC50=26.03 mg/mL),表明武夷名丛茶多糖是一种较好的羟基自由基清除剂,具有较强的抗氧化活性,不同名丛间茶多糖活性具有多样性。Five different Wuyi Ming Cong tea plant germplasm resources JM054 （＇JMH＇）, JM045 （＇JLH＇）, JM065 （＇LLX＇）, JM024 （＇YQL＇） and JM028 （＇YGY＇） were collected. Then the contents of flavonoids, free amino acids, soluble sugar, tea polyphenols and other major biochemical components were measured. Tea polysaccharides （TPS） of each sample were obtained. The contents of protein, neutral sugar, uronic acid, tea polyphenol of each tea polysaccharide were measured. Then Vc was set as a positive control, and the method of D-deoxyribose-iron system were adopted to compare the scavenging activity of hydroxyl radical （. OH） by TPS. The results showed that the contents of soluble sugar in different Wuyi Ming Cong tea plants were significantly different and the free amino acids and tea polyphenols even reached a very significant level, also there was a very significant difference in the contents of uronic acid and neutral sugar in TPS, it illustrated that there was a relatively strong diversity in biochemical composition of Wuyi Ming Cong tea plants and TPS in Wuyi Ming Cong tea plants. There was a significant difference of scavenging rate of hydroxyl radical by TPS in different Wuyi Ming Cong tea plants： ＇YGY＇ -TPS （IC5o=4.37 mg/mL）〉 ＇JMH＇ -TPS （IC5o=4.80 mg/mL）〉 ＇YQL＇- TPS （IC5o=6.21 mg/mL）〉 ＇JLH＇-TPS （IC5o=6.31 mg/mL）〉 ＇LLX＇-TPS （IC50=6.75 mg/mL）, all of them had a better scavenging effect than Vc （IC50=26.03 mg/mL）, indicating that TPS in Wuyi Ming Cong tea plants was a kind of good hydroxyl radical scavenger which possesses a strong antioxidant activity, and there was a relatively strong diversity of antioxidant activity of Wuyi Ming Cong tea plants.

关 键 词：武夷名丛 生化成分 茶多糖 羟基自由基 抗氧化活性 

分 类 号：S571.1[农业科学—茶叶生产加工]