PFGE与16S rDNA对阪崎克罗诺杆菌分型的研究  

Study on the molecular typing of Cronobacter sakazakii with pulsed-field gel electrophoreses and 16S rDNA

作　　者：柴云雷[1,2] 满朝新[3] 卢雁[3] 闫天文[1,2] 刘泳麟[1,2] 庞心怡[1,2] 李然[1,2] 姜毓君 

机构地区：[1]东北农业大学 [2]东北农业大学食品学院,哈尔滨150028 [3]东北农业大学国家乳业工程技术研究中心,哈尔滨150028

出　　处：《中国乳品工业》2014年第10期11-14,共4页China Dairy Industry

基　　金：国家科技支撑计划项目(2013BAD18B11,2012BAK17B04,2012BAD28B02);黑龙江省科研机构创新能力提升专项计划(YC13D005)

摘　　要：研究脉冲场凝胶电泳(PFGE)与16S rDNA序列分析在阪崎克罗诺杆菌分型中的应用。对分离自不同来源的10株阪崎克罗诺杆菌进行16S rDNA序列扩增及测序,并构建系统发育树进行聚类分析。再利用限制性内切酶Xba I对这10株菌酶切后,进行PFGE电泳分型。16S rDNA序列分析显示,所有分离菌株与ATCC标准菌株在同一系统发育分支下,其序列相似性达到98.37%,并且不能进一步分型。而PFGE分型结果显示,10株分离菌株和2株标准菌株共分成11种PFGE基因型,说明PFGE的分型能力明显优于16S rDNA序列分析,部分菌株的基因型与分离来源具有一定的相关性,所以PFGE分型方法可用于阪崎克罗诺杆菌分子分型及溯源的研究。To study the Cronobacter sakazakii parting with pulsed-field gel electrophoresis and 16 S rDNA sequence analysis. 10 strains of C. sakazakii separated from different sources were analyzed by 16 S rDNA sequence amplification and sequencing, and phylogenetic tree were built for clustering analysis. And the 10 strains were digested using restriction enzymes Xba I for PFGE classification and clustering analysis. 16 S rDNA sequence analysis showed that all isolates were in the same phylogeny branch as the ATCC standard strains, the sequence similarity was 98.37% and cannot be further classified. The PFGE classification results showed that 10 strains of isolates and 2 standard strains were divided into 11 kinds of PFGE genotype and thus PFGE has better classification capacity than 16 S rDNA sequence analysis. The genotype and separating source of some isolates has certain relevance, so PFGE classification method can be used for C. sakazakii molecular classification and traceability.

关 键 词：阪崎克罗诺杆菌 脉冲场凝胶电泳 16SrDNA 

分 类 号：TS207.4[轻工技术与工程—食品科学]