乙醇对重组毕赤酵母表达猪α干扰素的性能及其碳源代谢关键酶活性的影响  

Effect of ethanol accumulation on porcine interferon- α production by Pichia pastoris and activities of key enzymes in carbon metabolism

作　　者：丁健[1] 高敏杰[1] 侯国力 梁克学 史仲平[1] 于瑞嵩[2] 李震[2] 

机构地区：[1]江南大学生物工程学院、工业生物技术教育部重点实验室,江苏无锡214122 [2]上海农业科学院畜牧兽医研究所,上海201106

出　　处：《食品工业科技》2014年第21期150-153,158,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(#31301408);江苏省自然科学基金(#BK20130122)

摘　　要：本研究将细胞培养末期乙醇积累浓度低于2g/L的两个批次作为对照,探讨了长时间（大于4h）和瞬时的高乙醇浓度（10g/L以上）对猪α干扰素表达性能以及碳源代谢关键酶活性的影响。结果表明,长时间的高乙醇环境会大幅度降低甘油以及甲醇代谢过程中关键酶的比活性,最终导致猪α干扰素表达水平比对照组降低约4~12倍;高乙醇浓度的瞬时冲击后,上述关键酶比活性也会有一定程度的降低,猪α干扰素表达水平降低约8倍。In the present study, taking two batches with low ethanol accumulation under 2g/L as control group, effect of longterm （ 〉 4h） and instantaneous high ethanol concentration （ 〉 10g/L） on porcine interferon production and activities of key enzymes in carbon metabolism was discussed. As a result, long-term high ethanol concentration severely deteriorated specific activities of the enzymes, and plFN-α expression level was decreased about 4-12 folds. Similarly, instantaneous high ethanol concentration also deteriorated specific activities of the enzymes,and pIFN-α expression level was decreased about 8 folds.

关 键 词：毕赤酵母 乙醇积累 猪Α干扰素 酶活 

分 类 号：TS201.3[轻工技术与工程—食品科学]