表面活性剂辅助酶法提取黄芪黄酮的工艺优化  

Process optimization of surfactant assisted enzyme extraction of Astragalus flavonoids

作　　者：王超[1] 杜冰[1] 谢蓝华[1] 张嘉怡[1] 林凤英[1] 杨公明[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品工业科技》2014年第23期227-231,共5页Science and Technology of Food Industry

基　　金：广东省科技计划农业攻关项目(2012B020311006)

摘　　要：以乙醇为提取剂,采用表面活性剂辅助纤维素酶解法预处理从黄芪中提取黄酮,在单因素实验结果基础上,利用Box-Behnken的中心组合实验设计,运用响应面分析法对影响黄芪黄酮得率的主要因素(溴化十六烷基三甲胺(CTMAB)添加量、提取温度、乙醇浓度)进行优化,得出黄芪黄酮的最佳提取工艺条件为:CTMAB添加量0.37%,乙醇浓度80%,提取温度79℃,在此条件下黄酮得率实测值为4.192%,理论值为4.287%,实测值与理论值相差较小。The flavonoids was extracted by surfactant-assisted enzymatic pretreatment with alcohol as the solvent from Astragalus.Experiment factors and levels were firstly selected by one-factor tests.According to the Box- Benhnken center-united experimental design principles,the method of response surface analysis was adopted to optimize extraction of flavonoids .The results showed that the optimum conditions were.addition of CTMAB 0.37%, concentration of ethanol 80% ,temperature 79℃.Yield of that optimized procedure was 4.192% ,which was close to the predicted value of 4.287%.

关 键 词：黄芪 黄酮 表面活性剂 纤维素酶 提取 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]