黑曲霉利用纤维素发酵生产柠檬酸培养基优化  

Medium optimization for citric acid production by Aspergillus niger with cellulose as carbon source

作　　者：周凤侠 孙科[2] 

机构地区：[1]中央农业广播学校徐州分校,江苏徐州221000 [2]徐州生物工程职业技术学院生物工程系,江苏徐州221006

出　　处：《发酵科技通讯》2014年第4期11-13,10,共4页Bulletin of Fermentation Science and Technology

基　　金：徐州生物工程职业技术学院2013年立项课题(2013B07)

摘　　要：以黑曲霉(Aspergillus niger)XSY0607作为生产菌株,利用纤维素作为碳源,通过液体深层发酵对其进行产柠檬酸研究。实验结果表明在7 L小型发酵罐中接入种龄为1 d的种子培养液、30°C下通风搅拌培养5 d的培养条件下,适合产酸的条件为:水稻秸秆粉6%、蛋白胨5%、聚乙二醇0.1%、初始pH值为6.5。该试验结果为黑曲霉XSY0607菌株以纤维素作为原料发酵生产柠檬酸的工业化生产和应用提供一定的参考依据。Fermentive production of citric acid with cellulose as carbon source by Aspergillus niger XSY0607 was studied. The results showed that when the seed culture （the inoculum age was 1 d） was inoculated in to a 7 L fermentor, the optimal culture components for citric acid production were 6% of rice straw powder, 5% of peptone, 0.1% of polyethylene glycol, with the initial pH A spergillus 6.5. These results laid foundations niger XSY0607 with cellulose as raw for the industrial production of citric acid by material.

关 键 词：黑曲霉XSY0607 水稻秸秆 培养基 液体深层发酵 

分 类 号：TQ92[轻工技术与工程—发酵工程]