响应面法优化Nisin发酵的培养条件  

Optimize culture conditions of streptococcus thermophilus for fermentation producing nisin by response surface methodology

作　　者：贺小贤[1] 郑茂奎 刘欢[1] 彭光杰[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2014年第6期119-123,133,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金青年项目(31301059);陕西省西安市科技创新支撑计划项目(NC1120(3))

摘　　要：为了提高嗜热乳链球菌发酵生产Nisin的效价,以MRS为发酵培养基,以金黄色葡萄球菌为效价检测指示菌,以Nisin效价为考察指标,在单因素实验的基础上,采用响应面分析法优化发酵初始pH、接种量以及玉米浆添加量.结果表明影响Nisin效价的最大因素是玉米浆添加量,其次是发酵初始pH,接种量影响最小.最佳初始pH6.60,接种量4.80%,玉米浆添加量2 560%,在此条件下Nisin的效价可达1 602.77±6.38IU/mL,较优化前提高了18.07%,其实验结果与模型预测值拟合良好,说明通过响应面试验设计对Nisin发酵条件的优化是有效的.In order to improve fermentation titer unit of Nisin ,the MRS was used as the fermentation medium by streptococcus thermophilus and titer unit of Nisin to Staphylococcus aureus which is used as indicator bacteria serve as detection index of titer unit of Nisin .On the basis of single factor experiment ,optimization of fermentation initial pH ,inoculation amount of Streptococcus thermophilus and corn steep liquor content for producing Nisin by using response surface analysis methodology .The experiment results showed that the optimal fermentation condition was initial pH 6 .60 ,inoculation amount of 4 .80% and corn steep liquor content of 25 .60% ,and corn steep liquor content is the maximum factor for affecting the fermentation titer unit of Nisin and inoculation is the minimal factor .Under these conditions , the Nisin titer unit was increased by 18 .07% and reached up to 1 602 .77±6 .38 IU/mL which is consistent with the predicted value .These show that it is effective for optimizing culture conditions of Nisin by response surface methodology .

关 键 词：响应面法 NISIN 培养条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]