黄酒多糖对S180荷瘤小鼠肿瘤抑制及免疫增强作用  

Effects of polysaccharides from Chinese rice wine on immunity correlating cytokines of immunodeficient mice

作　　者：沈赤[1,2] 毛健[1,2,3] 陈永泉[1] 孟祥勇[1,2,3] 艾斯卡尔.艾拉提 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]国家黄酒工程技术研究中心,浙江绍兴312000

出　　处：《食品工业科技》2014年第24期346-350,共5页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)(2013AA102203-06);国家自然科学基金(31271839)

摘　　要：研究黄酒多糖对S180荷瘤小鼠肿瘤抑制及免疫增强作用。随机分成正常空白组(Control)、阴性对照组(生理盐水组,NC)、阳性对照组(环磷酰胺组,CY)、高、中、低剂量组(HCRWP、MCRWP、LCRWP),每组10只。接种小鼠S180肿瘤腹水24h后,每天灌胃0.01m L/g,连续灌胃10d,其中Control和NC分别以等量生理盐水代替。空腹称重并脱臼处死小鼠,测定免疫器官指数和抑瘤率,并采用免疫组化法检测S180瘤组织中Ki-67和Cyclin D1的含量。结果黄酒多糖可以明显增加S180荷瘤小鼠脾指数和胸腺指数,抑瘤率随黄酒多糖剂量的增加而增加;并且能阻碍Ki-67和Cyclin D1表达,抑制肿瘤细胞生长。由此说明,在实验范围内,各剂量黄酒多糖均能改善荷瘤小鼠的免疫功能,抑制肿瘤增殖。The effects of polysaccharides from Chinese rice wine on immunity correlating cytokines of immunodeficient mice were studied. Sixty mice were divided into six groups.blank control,negative control （NC）, cyclophosphamide （CY）, polysaccharides from Chinese rice wine （50,100,200mg/kg）. The S 180 ascites sarcoma mice models were established by transplanting the ascites sarcoma cell suspension in the mice left anterior subcutaneous part. After 24 hour,the mice in CY group and CRWP groups were peritoneally fed with CY and polysaccharides from Chinese rice wine and by gavage once a day for 10 days,and that in blank control group and negative control group were fed with same volume physical saline. And the effects of polysaccharides from Chinese rice wine on immunity organ was evaluated by immune organ index,and that on S180 cells in vivo was evaluated by the tumor weight of tumor-beating mice and the tumor inhibition rate. The expression of Ki-67 and Cyclin D1 was determined by immunohistochemistry. This results indicated that polysaccharides from Chinese rice wine might increase the index of spleen and thymus and the anti-tumor rate for S180-bearing mice. It indicated that the higher doses of polysaccharides from Chinese rice wine,the better improvement effect of immune organ index and the expression of Ki-67 and Cyclin D1 protein. Polysaccharides from Chinese rice wine had the antitumor effects probably by improving the immune function of immunodeficient mice.

关 键 词：黄酒多糖 S180瘤 免疫抗原 免疫器官指数 

分 类 号：TS262.4[轻工技术与工程—发酵工程]