平菇P1培养条件优化及其黄曲霉毒素降解酶的初步分离  

Optimization of the Culture Condition and Preliminary Separation of Aflatoxin Degrading Enzymes of the Strain Pleurotus ostreatus P1

作　　者：周露[1] 王会娟[1] 邢福国[1] 魏丹丹[1] 刘阳[1] 

机构地区：[1]中国农业科学院农产品加工研究所/农业部农产品加工综合性重点实验室,北京100193

出　　处：《核农学报》2014年第9期1625-1631,共7页Journal of Nuclear Agricultural Sciences

基　　金：公益性行业(农业)科研专项"粮油真菌毒素控制技术研究"(201203037);国家重点基础研究发展计划"主要粮油产品储藏过程中真菌毒素形成机理及防控基础"(2013CB127805);中央级公益性科研院所基本科研业务费专项(0032012020)

摘　　要：前期筛选获得了可降解黄曲霉毒素的平菇P1,为进一步提高其降解能力,通过优化平菇P1的培养条件,确定在培养温度30℃、转速200 r·min-1、培养基pH值6.0的条件下培养10d时,平菇P1发酵液降解黄曲霉毒素B1的效果最好,此时790μL平菇P1发酵液可以将1 000 ng黄曲霉毒素B1降解到175.7 ng,降解率达到82.43%。除了可以高效降解黄曲霉毒素B1,平菇P1发酵液还可有效降解黄曲霉毒素B2、G1和G2。平菇P1发酵液对黄曲霉毒素的降解是由多种酶参与的,它们之间存在累加效应。这一研究结果将为典曲霉毒高效降解酶的研制提供基础。In order to improve the degradation ability of the strain Pleurotus ostreatus P1, the culture condition of this strain was optimized, the best condition was as follows： culture temperature was 30 ℃ , incubation speed was 200 r. min^-1, the pH of MSM medium was 6.0, and culturing the strain for 10 days. Under the Optimized condition, 1000 ng AFB1 can be degraded to be 175.7 ng by 790 μL Pleurotus ostreatus P1 fermentation and the degradation rate reached to 82.43%. Besides degrading AFB1, Pleurotus ostreatus P1 fermentation can also effectively degrade AFB2, AFG1 and AFG2. AFB1 degradation was made by multiple enzymes in Pleurotus ostreatus P1 fermentation with accumulative effects. The results will provide useful information for exploring aflatoxin-degradation eyzymes.

关 键 词：平菇 黄曲霉毒素 培养条件优化 降解酶 分离 

分 类 号：S646.14[农业科学—蔬菜学]