蜂胶葡萄酒中8种有机酸RP-HPLC分析方法的建立  

Determination of Aliphatic Organic Acids in Propolis Wine by RP-HPLC

作　　者：丁青芝[1] 谷耀光 周艺诗 杨丽[1] 莫燕芳[1] 马海乐[1] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013

出　　处：《食品工业》2014年第12期279-283,共5页The Food Industry

基　　金：国家863计划重点项目(2013AA100203);江苏省博士后基金(1302013A);国家自然科学基金(31071502);江苏大学高级人才启动基金(10JDG031);江苏高校优势学科建设工程资助项目(PAPD2012);江苏大学大学生科研立项(11A351;11A345;12A003;12A012);江苏大学大学生创新计划(201310299041W;201310299045W;201310299046W)

摘　　要：研究了HPLC测定蜂胶葡萄酒中8种有机酸的快速检测方法。优化得到色谱条件：色谱柱,Agilent Eclipse XDB-C18（5μm,4.6 mm×250 mm）;流动相为15 mmol/L KH2PO4（用磷酸调p H至2.1）-甲醇（98∶2）,流速0.8 m L/min;紫外检测波长205 nm和243 nm,柱温30℃,进样量10μL,等度洗脱。结果表明,8种有机酸的线性关系良好,相关系数在0.999 1-1之间;精密度、稳定性的RSD都低于3.08%;平均回收率93.60%-109.00%,定量限为0.001 2-0.015 mg/m L之间,各组分相对标准偏差（n=6）为0.23%-3.08%。该方法简便,具有较高的准确度和精密度,可应用于蜂胶葡萄酒中有机酸的检测。For the establishment of propolis wine quality evaluation system, 8 kinds of organic acid was studied. A method was developed for simultaneous determination of 8 kinds of organic acids by reversed phase high performance liquid chromatography（RP-HPLC）, according to characteristics of column and traits of wine. The optimal condition was contained that Agilent Eclipse XDB-C18（5 μm, 4.6 mm×250 mm）, a mobile phase gradient elution with 15 mmol/L KH2PO4（p H was adjusted to 2.1 with phosphoric acid）-methanol（98∶2）, flow rate of 0.8 m L/min, detection wavelength 205 nm and 243 nm, column temperature 30 ℃. The quantified analysis of external standard calibration curves was used. There were good linear correlations, and the correlation coefficient were 0.999 1-1. The detection limits were 0.001 2-0.015 mg/m L. The recoveries（n=6） ranged from 93.60% to 109.00%, and the relative standard deviations（n=6） were from 0.23% to 3.08%. The method was convenient, rapid, accurate, sensitive and applicable to determine organic acids in wine.

关 键 词：高效液相色谱法 有机酸 葡萄酒 蜂胶 

分 类 号：O657.72[理学—分析化学] TS262.6[理学—化学]