山西老陈醋中川芎嗪测定及特征分析研究  

Determination of the ligustrazine in the Shanxi mature vinegar and the research on characteristic sizes

作　　者：朱丽[1] 樊垚[1] 曾泳艇[1] 熊岑[1] 郑彦婕[1] 

机构地区：[1]深圳市计量质量检测研究院,深圳518131

出　　处：《食品科技》2014年第12期294-298,共5页Food Science and Technology

基　　金：质检行业公益性科研专项(2012104019-6)

摘　　要：研究开发了测定山西老陈醋中川芎嗪含量的高效液相色谱方法,具体条件为：流动相：水（1%乙酸＋0.05%三氯乙酸）-甲醇（45：55,体积比）,流速：1.0 m L/min;柱温：30℃;检测波长：297 nm,进样体积10μL,川芎嗪外标法定量。结果表明,该方法回收率为77.3%-98.9%,RSD为1.1%,整体方法操作简单,回收率及精密度较好。研究采集了地理标志保护产品山西老陈醋和非地理标志陈醋（非地标山西醋和其他对照陈醋）,对其含有的特征指标川芎嗪进行测定及比较,研究表明地理标志山西老陈醋与非地标陈醋中川芎嗪含量差异明显,且地理标志山西老陈醋中川芎嗪含量与陈酿年份存在一定的正比例关系,从而明确了川芎嗪作为特征指标对于地理标志产品山西老陈醋产品鉴定评价的意义。A quick, accurate and efficient high performance liquid chromatography（HPLC）method was developed for the determination of ligustrazine in the Shanxi mature vinegar. The chromatographic separation is performed at 30 ℃ in the isocratic elution mode using a mobile phase composed of methanol and water（1% acetic acid＋0.05% trifl uoroacetic acid, 55：45, V/V） with the flow rate of 1.0 mL per min in the detective wavelength of 297 nm. Each the sample injection volume was 10 μL. Additionally, trifluoroacetic was used for the quantifi cation as the external standard. It is indicated that the average recovery rate ranges from 77.3% to 98.9% with the relative standard deviation（RSD）of 1.1%. The HPLC method demonstrates to be smart with high recovery rate and of high precision. A series of labeled China Protect of Geographical products of Shanxi aged vinegar and normal mature vinegar（Shanxi vinegar and other non landmark vinegar） were collected in this study. Meanwhile, the content of Ligustrazine was measured and compared between and within the different kinds of samples. Significant difference was found in the concentration of ligustrazine between the China Protect of Geographical products ofShanxi aged vinegar and unlabeled vinegar. It was shown that among the labeled vinegar products, there exists a positive relationship between the content and the vintage years. Ligustrazine was proved to be an important evaluation index of identifying and judging the China Protect of Geographical products of Shanxi aged vinegar.

关 键 词：川芎嗪 山西老陈醋 高效液相色谱 特征指标 

分 类 号：TS264.22[轻工技术与工程—发酵工程]