土壤中产脂肪酶菌株的分离及浓缩培养  

ISOLATION AND CONCENTRATED CULTIVATION OF LIPASE-PRODUCING STRAINS FROM SOIL

作　　者：李霞[1] 刘尚军[1] 岳春[1] 曾虎[1] 

机构地区：[1]南阳理工学院生物与化学工程学院,河南南阳473000

出　　处：《河南工业大学学报（自然科学版）》2014年第6期56-61,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：江苏省生物质绿色燃料与化学品重点实验室开放基金资助项目(JSBGFC12008)

摘　　要：从食用油污染的土壤中筛选得到1个产脂肪酶较多的酵母菌株,测得其脂肪酶活力为8.30 U/m L.用正交试验法对该菌株的最佳发酵条件进行了优化.结果表明,最佳培养基成分为:1.50%蛋白胨、1.20%葡萄糖、10.00%橄榄油乳化液、0.5%酵母膏、KH2PO40.65%、K2HPO40.20%、Mg S04·7H2O 0.02%;最佳产酶培养条件为:温度30℃,发酵的初始p H 7.5,接种菌量为2.0%,摇床转速180 r/min,培养20 h后测定脂肪酶酶活力达到33.19 U/m L,是优化前的4倍.在菌株培养20 h时采用微孔滤膜过滤二次培养,结果表明在培养24 h时菌数达到最大;培养28 h后脂肪酶活力达到峰值86.08 U/m L,较未采用微孔滤膜过滤二次培养提高160%,为初始脂肪酶活力的10.4倍,达到浓缩培养要求.We obtained a yeast strain with high lipase producing ability from soil contaminated by edible oil, and determined the lipase activity （8.30 U/mL）. The optimum fermentation conditions were optimized through orthogonal experiments. The results showed that the optimum composition of culture medium comprised： peptone 1.5%, glucose 1.2%, olive oil emulsion 10%, yeast extract 0.5%, KH2PO4 0.65%, K2HPO4 0.20%, and MgSO4· 7H2O 0.02%. The optimum lipase-producing conditions were as follows： temperature 30℃ ,initial pH 7.5, inoculant dosage 2.0% ,and speed of shaking table 180r/min. The lipase activity reached 33.19 U/mL after cultivation for 20 hours, which was improved by three times. After cultivation for 20 hours, the strain was filtered by a micro-filtration membrane and cultured for the second time. The results showed that the microbial content reached the highest value after cultivation for 24 hours; the lipase activity reached the peak value （86.08 U/mL） after cultivation for 28 hours,which was improved by 160% than that without filtration and secondary cultivation; and the peak lipase activity was 10.4 times the initial value,and could meet the requirement for concentrated cultivation.

关 键 词：酵母菌 脂肪酶 微孔滤膜 浓缩培养 

分 类 号：TS201.3[轻工技术与工程—食品科学]