水分含量对L-抗坏血酸-L-半胱氨酸Maillard反应体系中挥发性产物的影响  

Effect of Water Content on Generation of Volatile Compounds from Maillard Reaction of L-Ascorbic Acid and L-Cysteine

作　　者：谭志伟[1] 余爱农[1] 刘应煊[1] 

机构地区：[1]湖北民族学院化学与环境工程学院,湖北恩施445000

出　　处：《食品科学》2014年第10期132-138,共7页Food Science

基　　金：国家自然科学基金地区科学基金项目(31360408);湖北省教育厅自然科学研究项目(B2011901)

摘　　要：采用顶空固相微萃取-气相色谱-质谱联用技术对L-抗坏血酸-L-半胱氨酸反应体系在不同水分含量下的产物进行鉴定,共鉴定出包括噻吩、硫杂环、噻唑、硫醇、呋喃和硫醚等在内的62种化合物,其中23种噻吩化合物和13种硫杂环化合物为2种主要挥发性产物。结果表明,水分含量对硫杂环类化合物和噻吩类化合物影响明显,而对其他几类挥发性产物的影响不大。随水分含量的增加,硫杂环类产物中3,5-二甲基-1,2,4-三硫环戊烷2种异构体和4,6-二甲基-1,2,3,5-四噻烷3种产物在水分含量为55%时有极大值;2,4,6-三甲基-1,3,5-三噻烷的2种异构体随水分含量的增加而缓慢下降,3-甲基-1,2,4-三噻烷在水分含量为45%有极小值。噻吩类产物中,噻吩、2-甲基噻吩、[3,2-b]并二噻吩、2-甲基[2,3-b]并二噻吩和2-甲基-3-巯基噻吩分别在水分含量为75%、55%、85%、65%和15%时达到最大值。The identification of volatile compounds generated from the Maillard reaction of L-ascorbic acid and L-cysteine under different water content conditions was performed using headspace solid-phase microextraction-gas chromatographymass spectrometry （HS-SPME-GC-MS）. Sixty-two volatile compounds were identified, including thiophenes, sulfur-containing heterocyclic compounds, thiazoles, thiols, furans and thioethers. Meanwhile, 23 thiophenes and 13 sulfur-containing heterocyclic compounds were the major volatile compounds in the Maillard reaction products. The effect of water content on the generation of volatile compounds was investigated. The results showed that water content had significant effect on the formation of sulfur-containing heterocyclic compounds and thiophene compounds, but had little effect on other volatile products. Among these sulfur-containing heterocyclic products, two isomers of 3,5-dimethyl-1,2,4-trithiolane and 4,6-dimethyl-1,2,3,5-tetrathiane had the maximum concentration when the water content was 55%. The concentration of two isomers of 2,4,6-trimethyl-1,3,5-trithiolane decreased with increasing water content, and 3-methyl-1,2,4-trithiane had a minimum level at the water content of 45%. However, thiophene products, such as thiophene, 2-methylthiophene, thieno[3,2-b]thiophene, 2-methylthieno[2,3-b]thiophene and 2-methyl-3-thiophenethiol reached a maximum level at the water contents of 75%, 55%, 85%, 65% and 15%, respectively.

关 键 词：MAILLARD反应 抗坏血酸-半胱氨酸 水分含量 噻吩 硫杂环化合物 

分 类 号：TS201.7[轻工技术与工程—食品科学]