压力耐受啤酒酵母的筛选及其RT-PCR研究  

Screening and RT-PCR analysis of stress resistant brewing yeast

作　　者：李欣儿[1,2] 王金晶[1,2] 李崎[1,2] 

机构地区：[1]江南大学教育部工业生物技术重点实验室,江苏无锡214122 [2]江南大学酿酒科学与工程研究室,江苏无锡214122

出　　处：《食品与发酵工业》2014年第8期18-23,共6页Food and Fermentation Industries

基　　金：江苏高校优势学科建设工程资助项目(PAPD);111引智计划;教育部新世纪人才支持计划项目资助(No.NCET-10-0453);国家高技术研究发展计划(863计划;No.2012AA021303);国家高技术研究发展计划(863计划;No.2013AA102106-03);国家自然科学基金(31271919);国家自然科学基金(31301539)

摘　　要：为了提高啤酒酵母对发酵环境中存在的多种压力的耐受性,采用紫外线对啤酒工业生产菌株Y-1进行诱变。经过米卡芬净平板的初筛和压力平板的复筛,获得了2株压力耐受性明显增强的啤酒酵母MR0-8和MR1-2。与出发菌株Y-1相比,突变菌株MR0-8和MR1-2发酵结束细胞活力分别提高了19%和34%。进一步对出发菌株Y-1和突变菌株MR1-2的压力耐受途径的基因进行了RT-PCR分析。结果显示:在正常条件下,MR1-2的压力应答基因及细胞完整性基因的表达量比出发菌株Y-1都有不同程度的提高;在乙醇压力和高渗透压压力下,菌株Y-1的大部分相关基因都上调,而MR1-2的相关基因则表现出复杂的应答反应。In order to improve resistance to various stresses during fermentation of brewing yeast, UV mutagene- sis was carried out on industry brewing yeast Y-1. Strain MR0-8 and MR1-2 with obviously improved stress tolerance were isolated on micafungin plate and stressful plates. Compared with the origin strain Y-1 , the viability of strain MR0-8 and MR1-2 at the end of fermentation were improved by 19% and 34% , respectively. Further RT-PCR analy- sis indicated that the expression of genes for stress response and genes in cell wall integrity pathway were higher in MR1-2 than those in Y-1. In response to ethanol and hyperosmolarity stress, most responsive genes were unregulated in Y-1 , while the performance of these genes was more complicated in MR1-2.

关 键 词：啤酒酵母 紫外诱变 压力耐受性 米卡芬净 

分 类 号：TS262.5[轻工技术与工程—发酵工程]