发酵生产γ-聚谷氨酸的补料策略  

The production of poly-γ-glutamic acid(γ-PGA) through fed batch fermentation strategy

作　　者：王青龙[1] 陈桂光[1] 郑双凤[1] 唐真[1] 粱智群[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004

出　　处：《食品与发酵工业》2014年第9期52-56,共5页Food and Fermentation Industries

基　　金：广西研究生教育创新计划资助项目(YCBZ2012004);2013年广西大学亚热带农业生物资源保护与利用国家重点实验室研究课题;2013年广西大学微生物及植物遗传工程教育部重点实验室主任基金课题

摘　　要：采用补料分批发酵方法对Bacillus subtilis GXA-28摇瓶发酵生产γ-聚谷氨酸的初始葡萄糖和谷氨酸钠浓度、补料时间、补料配方等进行了研究。确定最优补料时间为17 h,补料配方:葡萄糖20 g/L,谷氨酸5 g/L,KH2PO40.5 g/L、MgSO40.1 g/L。在优化的补料条件下,γ-聚谷氨酸产量由16.24 g/L提高至24.36 g/L,较分批发酵提高了50%;生产强度由0.74g/(L·h)提高至0.87 g/(L·h)。研究结果表明,采用分批补料发酵方法能提高γ-聚谷氨酸生产率,实验方法可给γ-聚谷氨酸中试研究提供参考。In this research, the initial concentration of glucose and sodium glutamate, feeding time, feeding for- mulation of γ-PGA produced by flask-shaking fermentation of Bacillus subtilis GXA-28 using fed batch culture were in- vestigated. The suitable feeding time was 17 h. Feeding formula was as followes： Glucose 20 g/L, glutamic acid 5 g/ L, KH2PO4 0.5 g/L, MgSO4 0. lg/L. Under optimal feeding conditions, the production of poly-γ-glutamic acid in- creased to 24.36 g/L, which was 50% higher than that from batch fermentation. Intensity of production increased from 0.74 g/（L·h） to 0. 87 g/（L·h）. The results showed that the fed-batch fermentation strategies had a signifi- cant effect on the production of γ-poly glutamate and gave a reference for γ-PGA pilot study.

关 键 词：Γ-聚谷氨酸 补料分批发酵 枯草芽孢杆菌 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]