杏鲍菇污染菌的分离、鉴定及其特性  

Isolation, Identification and Characterization of Contaminant Bacteria from Pleurotus eryngii

作　　者：姚璐晔[1] 沈金波[1] 戴璐[1] 裴丹丹[1] 孙丹凤[1] 冀宏[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《食品科学》2014年第9期213-217,共5页Food Science

基　　金：苏州应用基础研究项目(SYN201007;SYN201212);江苏省高等学校大学生实践创新计划项目(201210333014);常熟理工学院重点毕业论文资助项目(LG27)

摘　　要：从夏季被细菌污染的杏鲍菇上分离纯化得到2株病害菌(CTE722-B、CTE722-C),结合显微结构和生理生化实验结果,以及对它们16S rDNA序列的分析确定这两株菌的系统发育地位;通过抑菌圈实验考察几种工厂常用消毒剂对病害菌的抑菌影响。结果表明:菌株CTE722-B鉴定为恶臭假单胞菌属(Pseudomonas putida),CTE722-C为边缘假单胞菌属(Pseudomonas marginalis)。消毒剂菇保、甲酚皂、苯扎溴铵消毒液在短时间内对这两种细菌都有较好的抑菌效果;但因其具有挥发性,故防治效果不理想。从污染菇体分离得到的2株病害菌CTE722-B和CTE722-C被鉴定为假单胞菌属,因常用消毒剂只在短时间内对其有抑菌性,防治效果较差。This study aimed to identify and characterize the pathogenic bacteria which caused pollution in Pleurotus eryngii. Two strains named CTE722-B and CTE722-C were isolated from Pleurotus eryngii polluted in summer collected from a mushroom factory. According to their microstructure, physiological, biochemical properties and 16S rDNA sequences, the phylogenetic positions of the two strains were defined. By measuring the diameter of inhibition zone, the efficacy of sanitizers commonly used in the food industry against them was investigated. The results showed that CTE722-B and CTE722-C were identified as Pseudomonas putida and Pseudomonas marginais, respectively. Although the growth of two strains was restrained within a short time after adding dichloroisocyanuric acid sodium salt, cresol soap or benzalkonium bromide into the medium, the antibacterial efficacy of these sanitizers was poor due to the high volatility.

关 键 词：杏鲍菇 病害菌 假单胞菌 特性 

分 类 号：Q936[生物学—微生物学]