酸性电解水处理后南美白对虾贮藏过程中肠道微生物的多样性变化  

Changes of acidic electrolyzed water on intestinal microflora diversity of Penaeus vannawei during storage

作　　者：付娇娇[1,2,3] 彭织云[1,2,3] 刘海泉[1,2,3] 孙晓红[1,2,3] 潘迎捷[1,2,3] 赵勇[1,2,3] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心,上海201306 [3]农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306

出　　处：《食品工业科技》2015年第4期344-347,356,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(31271870);上海市科学技术委员会部分地方院校能力建设项目(11310501100);上海市科学技术委员会科技创新行动计划项目(12391901300);上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号)

摘　　要：肠道微生物是引起水产品腐败变质的主要污染源之一。酸性电解水(AEW)作为新型环保消毒剂,因其高效广谱杀菌的特点,已逐渐被应用于水产品安全控制方面并且日益受到人们的重视。本研究采用变性梯度凝胶电泳技术,分析了经自来水(TW)与酸性电解水分别处理的南美白对虾(Penaeus vannawei)于0、4、25℃贮藏过程中肠道微生物多样性的变化规律。结果表明,与对照相比,经酸性电解水处理后的样品肠道微生物多样性在贮藏过程中有所减少。因此,酸性电解水能明显改变肠道微生物的多样性,为其保障水产品质量安全提供了理论参考依据。Intestinal microflora was considered as one of the major sources to cause the corruption of aquatic products. As a new- type environmental disinfectant, acidic electrolyzed water（ AEW） had gradually been applied to control the security of aquatic products,which was receiving more and more attentions due to its efficient broad-spectrum sterilization. In this paper,PCR-DGGE technology was used to analyze the variation of intestinal microflora diversity in Penaeus vannamei stored at 0℃,4℃ and 25℃,which were treated by tapwater（ TW） and AEW, respectively. Compared with TW group, samples under AEW showed a decreasing diversity of intestinal microflora during storage,which indicated that intestinal microflora diversity was significantly affected by AEW. The results would be used to provide a theoretical reference for the security of aquatic products.

关 键 词：南美白对虾 酸性电解水 肠道微生物 变性梯度凝胶电泳(DGGE) 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]