植物乳杆菌亚硝酸盐还原酶相关基因的研究  

Study on Lactobacillus plantarum Nitrite Reductase Gene

作　　者：何婷婷[1,2] 龚钢明[1] 高然[3] 

机构地区：[1]上海应用技术学院,上海201418 [2]上海师范大学,上海200234 [3]浙江理工大学,杭州310018

出　　处：《食品工业》2015年第2期191-194,共4页The Food Industry

摘　　要：植物乳杆菌具有降解亚硝酸盐的能力。根据已知四种类型亚硝酸盐还原酶蛋白序列的保守序列,利用互联网相关蛋白分析软件及生物信息学工具,查找到植物乳杆菌基因组上的亚硝酸盐还原酶疑似基因8个,克隆出全部基因序列,分别构建表达载体p ET-32a(+)-Ni Rs。将构建的质粒转入BL21(DE3)表达菌的感受态细胞中进行诱导表达,表达的目的蛋白,经亚硝酸盐还原酶总活性测定试剂盒检测,结果显示,其中HP蛋白具有显著还原亚硝酸盐的能力。Lactobacillus plantarum has the ability to degrade nitrite. According to four types of conserved protein sequences of known nitrite reductive in bacteria, using Interact-related protein analysis sottware and bioinformatics tools, putative Nirs genes was found in genome ofLactobacillus plantarum. Eight pairs of specific primers were designed and putative Nits genes were obtained by PCR amplification. All cloned gene sequences were inserted into pET-32a （＋） for constructing expression vector pET-32a （＋）-NiRs. The constructed plasmids were transformed into BL21 （DE3） expressing bacteria competent cells and they were inducted for gene expression. The expression protein was detected by total nitrite reductase activity assay kit. The results show wherein the HP protein has significantly the ability to reducing nitrite.

关 键 词：植物乳杆菌 亚硝酸盐还原酶 基因克隆 

分 类 号：TS201.3[轻工技术与工程—食品科学]