不同检测方法在抗菌肽抑菌效果评价的比较研究  

Comparison of Activity Assays for Antimicrobial Peptides

作　　者：潘晓倩[1] 成晓瑜[1] 张顺亮[1] 乔晓玲[1] 陈文华[1] 

机构地区：[1]中国肉类食品综合研究中心,肉类加工技术北京市重点实验室,北京100068

出　　处：《肉类研究》2014年第12期17-20,共4页Meat Research

基　　金："十二五"国家科技支撑计划项目(2012BAD28B09);北京市优秀人才培养资助项目(2013D001055000002)

摘　　要：目的:比较5种抗菌肽活性测定方法。方法:以金黄色葡萄球菌、溶血性葡萄球菌和枯草芽孢杆菌为实验菌株,研究牛津杯法、打孔法、滤纸片法、酶标比浊法和活菌计数法对不同质量浓度的抗菌肽抑菌活性评价的比较。结果:3种琼脂扩散定性抑菌实验中,打孔法灵敏度高,抑菌圈清晰规则,效果最好;2种定量抑菌实验中,活菌计数法结果判定更直观,灵敏度高,效果最好。结论:抗菌肽抑菌活性研究过程中,选用打孔法定性与活菌计数法定量相结合的方法,能够更全面、准确地反应其抑菌活性。Objective：To comparatively evaluate the performance of several antimicrobial peptide activity assays. Methods：Using Staphylococcus aureus, Staphylococcus haemolyticus and Bacillus subtilis as experimental strains, the antimicrobial activity of the antimicrobial peptide nisin at different concentrations was determined by Oxford cup method, punching method, filter paper method, microtiter plate assay and viable count method, respectively. Results： Among three agar-diffusion methods, punching method was the most sensitive, which provided clear and regular inhibition rings. Our comparison of two quantitative activity assays indicated that viable count method, showing high sensitivity and intuitive results, was better than microtiter plate assay. Conclusion： Combined application of punching method and viable count method allows comprehensive and accurate measurement of antimicrobial peptide activity.

关 键 词：抗菌肽 抑菌活性 测定方法 

分 类 号：TS207.4[轻工技术与工程—食品科学]