虹鳟肠道植物乳杆菌的分离及特性研究  

Isolation and characterization of indigenous Lactobacillus plantarum from rainbow trout intestine

作　　者：刘敏[1] 张英[1] 张琳琳[1] 蒋烨[1] 崔文[2] 姜艳萍[2] 乔薪瑗[2] 唐丽杰[2] 李一经[2] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2014年第12期24-30,35,共8页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(31372568);国家科技支撑计划(2012BAD25B02)

摘　　要：为筛选一株乳杆菌用于鱼类口服疫苗的活载体,研究从健康养殖的虹鳟肠道分离出58株形态特征不同的菌株,通过革兰氏染色、氧化酶、触酶反应筛选出18株革兰氏阳性杆菌,再通过生化反应筛选出5株表型不同的菌株。经16S r RNA序列同源性分析,5株均为植物乳杆菌。在8%Na Cl,0.5%胆盐,p H 3.0,7 g·L-1胰蛋白酶,10 g·L-1胃蛋白酶,60℃处理10 min时,几乎不影响菌体存活。5株菌对多种抗生素敏感,对革兰氏阳性及阴性细菌均有抑菌活性。通过荧光分子探针c FDA-SE标记细菌,分离株L1212在15 d的检测期内能在虹鳟肠粘膜定植,并有继续繁殖倾向。因此,植物乳杆菌L1212可作为益生菌应用于虹鳟口服疫苗载体及鱼类饲料添加剂开发。In order to select a suitable candidate probiotic for use as a live vaccine carrier, 58 strains of bacteria with different characters were isolated from the intestine of rainbow trout. By Gram staining, oxidase, catalase reaction, 18 Gram-positive bacilli were screened. Then by biochemical reactions, 5 strains with different phenotype were carried 16S rRNA sequence analysis, and the results showed that all of the 5 strains were Lactobacillus plantarum. A series of tolerance test was carried out, neither 7 g·L-1 trypsin, 10 g·L-1 pepsin, 8% NaCl, 0.5% bile, pH 3.0, nor exposure to 60℃ for 10 min significantly reduced their survival rates. Five strains exhibited sensitivity to various antibiotics, and inhibited the growth of both Gram-positive and Gram-negative bacteria. The intestinal epithelium of rainbow trout was colonized by cFDA-SE-labeled L. plantarum L1212 at 15 days post-inoculation, and have a potential to continue reproduction. The L. plantarum L1212 is a suitable candidate probiotic for use as a live vaccine carrier or feed additive for rainbow trout and other fishes.

关 键 词：植物乳杆菌 药物敏感性 抑菌活性 体内定植力 虹鳟 

分 类 号：S965.199[农业科学—水产养殖]