响应面法优化闪式提取杜香熊果酸及熊果酸性质研究  

Optimization of extraction conditions of Ursolic Acid from Ledum palustre L. by flash extraction using response surface analysis and properties analysis of Ursolic Acid

作　　者：张乔会[1] 逄锦慧 崔洁[1] 赵巧娇[1] 李建霞[1] 王建中[1] 

机构地区：[1]北京林业大学生物科学与技术学院,林业食品加工与安全北京市重点实验室,北京100083 [2]北京林业大学材料科学与技术学院,林木生物质化学北京市重点实验室,北京100083

出　　处：《食品工业科技》2015年第6期251-256,261,共7页Science and Technology of Food Industry

基　　金：林业公益性行业科研专项(201004081)

摘　　要：以杜香为原料,采取闪式提取辅助提取熊果酸,在单因素实验的基础上,对提取工艺进行响应面优化,最终得到优化的提取工艺参数为：闪式提取3min,提取温度为90℃,乙醇浓度为84%,料液比为1∶25,在此条件下实际测得的平均提取得率为1.52%。分析发现样品与标准品的红外吸收峰基本一致,样品含有熊果酸的典型基团。提取的杜香熊果酸在300-390℃温度范围内发生热解反应,失重率达70%以上。杜香熊果酸结晶度为64.3%,与熊果酸标准品基本一致。利用电镜扫描观察杜香熊果酸样品,发现其基本呈椭球形均匀分布。In this study, Ursolic Acid extracted from L. palustre with high extraction yield was successfully studied by the method of flash extraction. To improve the extraction yield of the Ledum Ursolic Acid in this study,a Box-Behnken design was employed based on four factor experiments. The four independent variables,namely extraction temperature,ethanol concentration ,solid-liquid ratio and flash extraction time on extraction yield of Ursolic Acid were evaluated by response surface methodology（RSM）. The results showed that the optimum conditions for extracting Ledum Ursolic Acid were as follows：the temperature 90℃,the ethanol concentration 84%,the solid-liquid ratio 1：25,and the flash extraction time 3min. Under the optimal conditions,the extraction yield achieved 1.52%.Furthermore,the groups and content of Ursolic Acid were investigated by FIR,extracts containing the typical group of Ursolic Acid standard,occurs of pyrolysis reaction in the temperature range of 300-390℃ ,the weight loss rate reached over 70% ,the crystallinity of Ursolic sample was 64.3% ,consistent with the ursolic acid standard, It was distributed in irregular ellipsoid by SEM.

关 键 词：杜香 熊果酸 闪式提取 响应面 热重 X-RD 

分 类 号：TS201.1[轻工技术与工程—食品科学]