离子对色谱-ELSD检测麦类中植酸的方法研究  

Determination of Phytic Acid in Triticeae Crops by Ion- Pair High- Performance Liquid Chromatography with ELSD

作　　者：袁建[1] 王艳[1] 范哲[1] 李倩[1] 何荣[1] 鞠兴荣[1] 

机构地区：[1]南京财经大学食品科学与工程学院,南京210046

出　　处：《中国粮油学报》2015年第3期128-133,共6页Journal of the Chinese Cereals and Oils Association

基　　金：863计划(2012AA101609)

摘　　要：建立了应用离子对色谱-蒸发光散射检测（IPC-ELSD）麦类作物中植酸含量的方法,并结合电喷雾质谱（ESI-MS）分析鉴定了各组分。色谱条件：C18色谱柱（5μm,4.6 mm×150 mm）;流动相：甲醇-水（体积比60∶40,含0.4%正戊胺,甲酸调节p H为4.5）;流速1.0 m L/min;柱温35℃。结果表明,植酸含量在0.25-5.0 mg/m L范围内线性关系良好（R2=0.999 1）,检测限为40μg/m L,平均回收率为90.44%,相对标准偏差为3.13%。该方法快速、准确,适用于麦类中植酸含量的测定。An ion- pair HPLC- ELSD method has been established through the research in the paper for determination of phytic acid in triticeae crops,which was combined with ESI- MS to identify the components. A C18 column（ 5 μm,4. 6 mm × 150 mm,column temperature 35 ℃） was used jointly with the mobile phase of methanol-water（ 60∶ 40,0. 4% n- pentylamine,formic acid p H 4. 5）,at a flow rate of 1. 0 m L / min. The calibration curve expressed a good linearity in the range of 0. 25 - 5. 0 mg / m L（ R2= 0. 999 1）. The detection limit was 40 μg / m L,and average recovery of 91. 11% with RSD of 5. 94%. The method is rapid and accurate,which is appropriate for the detection of phytic acid in triticeae crops.

关 键 词：离子对色谱 蒸发光散射检测 麦类 植酸 

分 类 号：O657.72[理学—分析化学]