棉籽肽的制备工艺、抗氧化活性及部分特性研究  

Preparation,antioxidant activity and partial properties of cottonseed peptide

作　　者：周向军[1] 张雪[1] 康桂琴 袁毅君[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001

出　　处：《中国油脂》2015年第3期30-35,共6页China Oils and Fats

基　　金：国家自然科学基金(81360619)

摘　　要：以水解度(DH)及DPPH·清除率为考察指标,利用单因素实验和正交实验探讨碱性蛋白酶酶解棉籽蛋白制备棉籽肽的最佳工艺条件。以VC为对照研究了棉籽肽的总还原力、对·OH及O-2·的清除作用及部分特性。结果表明,棉籽肽的最佳制备条件为:反应时间180 min,p H 9.5,温度55℃,底物质量浓度3.00 mg/m L,碱性蛋白酶加酶量50 U/m L。在最佳条件下,棉籽肽的DPPH·清除率为63.42%,DH为85.90%。棉籽肽的·OH及O-2·清除率均随其质量浓度增大而增强,半数抑制浓度(IC50)分别为0.044 mg/m L和0.046 mg/m L,且均强于VC。棉籽肽总还原力与其质量浓度呈剂量依赖关系,稍弱于VC。棉籽肽等电点约为p H 2,不同p H下,棉籽肽的·OH清除率均在60 min时达到最大,但在不同温度下,棉籽肽·OH清除率随时间变化不大,60℃时达到最大值。With degree of hydrolysis（ DH） and scavenging rate on DPPH· radical as indexes,the optimal enzymolysis conditions of cottonseed protein by alkali protease for preparation of cottonseed peptide were studied by single factor experiment and orthogonal experiment. With ascorbic acid（ VC） as control,the total reducing power and the scavenging effects of cottonseed peptide on hydroxyl radical and superoxide anion radical and its partial properties were also explored. The results showed that the optimal preparation conditions of cottonseed peptide were obtained as follows： reaction time 180 min,pH 9. 5,temperature 55 ℃,mass concentration of substrate 3. 00 mg / m L and dosage of alkali protease 50 U / m L. Under these conditions,the scavenging rate of cottonseed peptide on DPPH· radical and DH were 63. 42% and85. 90%,respectively. The scavenging rates of cottonseed peptide on hydroxyl radical and superoxide anion radical both increased with its mass concentration increasing,and half inhibitory concentrations（ IC50） of cottonseed peptide were 0. 044 mg /m L and 0. 046 mg /m L,respectively,both stronger than that of VC. Total reducing power of cottonseed peptide was dosage- independent and weaker than that of VC. Isoelectric point of cottonseed peptide was about 2. The scavenging rates of cottonseed peptide on hydroxyl radical all reached maximum in 60 min under different pH,while they were basically stable at different temperatures with time passing and reached the highest at 60 ℃.

关 键 词：棉籽肽 制备 抗氧化 酶解工艺 特性 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ931[轻工技术与工程—食品科学与工程]