SDS/正辛醇/异辛烷反胶束体系萃取葵花籽粕蛋白工艺研究  

SDS/isooctane/octanol reverse micellar extraction process of sunflower seed meal protein

作　　者：赵萍[1] 佐玲 郭求实[1] 王雅[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《食品工业科技》2015年第8期262-267,共6页Science and Technology of Food Industry

基　　金：甘肃省重点培育学科资助

摘　　要：利用SDS(十二烷基磺酸钠)/正辛醇/异辛烷反胶束体系对葵花籽粕蛋白进行萃取,探讨在超声辅助萃取下,W0、缓冲液p H、缓冲液浓度、料液比(g/m L)、温度、时间以及表面活性剂浓度等对蛋白质前萃取率以及后萃取率的影响。结果表明:前萃取工艺的最佳条件SDS浓度为0.07g/m L,料液比为1∶25,盐浓度为0.07mol/L,p H为6.5,W0=27,萃取时间为35min,温度为40℃;后萃取最佳工艺条件为:盐浓度为0.9mol/L、缓冲液p H为7.5、萃取时间为45min、萃取温度为35℃,在此条件下,蛋白质的前萃率为89.93%,后萃率为65.01%,蛋白质提取率为58.46%。Using reverse micelles of SDS（Sodium dodecyl sulfate）/isooctane/octanol extraction of sunflower seed protein, discussed in the ultrasound assisted extraction, study effect of W0, buffer p H, buffer concentration,solid-liquid ratio,temperature,time and surfactant concentration on the extraction rate of protein forward and backward extraction. The results showed that ： the optimum conditions of forward extraction process for the concentration of SDS was 0.07g/m L,the ratio of material to liquid was 1 ∶25（g/m L）,buffer concentration of0.07mol/L,p H6.5,W0=27,extraction time was 35 min,temperature was 40℃ condition for. Backward extraction optimum conditions were：the salt concentration was 0.9mol/L,p H value of buffer solution was 7.5,extraction time was 45 min,the extraction temperature was 35℃,under these conditions,the forward extraction rate of protein was 89.93%,backward extraction rate was 65.01%,the total extraction rate of protein was 58.46%.

关 键 词：反胶束 葵花蛋白 前萃取 后萃取 

分 类 号：TS255.6[轻工技术与工程—农产品加工及贮藏工程]