富硒茶中硒蛋白冻融法辅助提取工艺优化  

Process investigation on selenium-protein from selenium-enriched tea by repeated freezing and thawing alkali extraction

作　　者：王珺[1] 董文宾[1] 杨芙莲[1] 高二东[1] 缑敬轩[1] 

机构地区：[1]陕西科技大学,陕西西安710021

出　　处：《食品与机械》2015年第2期211-215,共5页Food and Machinery

基　　金：陕西省教育厅产业化培育项目(编号:14JF002);西安市科技局技术转移促进工程项目(编号:cxy1434)

摘　　要：为了使富硒茶茶渣中的硒蛋白具有更高的提取率,以单因素试验作为硒蛋白提取试验的基础,选择液固比、碱液浓度、提取时间和温度进行正交试验提取富硒茶中硒蛋白并对冻融法辅助提取硒蛋白的相关参数进行优化处理。结果显示,提取硒蛋白的最佳工艺条件为:Na OH溶液浓度0.10 mol/L,液固比60∶1(V∶m),提取温度70℃,提取时间3h,提取次数2次。在该条件下,硒蛋白粗品提取率为60.93%,硒蛋白粗品纯度为52.07%。并进一步利用乙醇沉淀法对所得硒蛋白粗品进行纯化,得到纯度为78.26%的硒蛋白,纯化过程中蛋白质的损失率为7.21%。Orthogonal test was carried on,choosing four factors： liquid-solid ratio,alkali concentration,extraction times and temperature,three levels to optimize the extracting technology of selenium-protein,on the basis of single factor experiments.Through this test,a series of related coefficients about alkaline extraction assisted by freeze-thaw method were optimized.The optimum extraction technological conditions were obtained as follows：Na OH solution 0.1 mol / L,solid-liquid ratio 60 ︰ 1（V ︰ m）,extracting temperature 70 ℃,extracting time 3 h,and extracting times twice,.Under these conditions,the extraction ratio of selenium-protein was 60.93%,the purity of which was 52.07%.The resulting crude protein was further purified by ethanol precipitation.The amount of selenium-protein in obtained product was 78.26%,and the loss rate of protein during purification was7.21%.

关 键 词：富硒茶 硒蛋白 冻融 碱提法 

分 类 号：TS272.5[农业科学—茶叶生产加工]