响应面法优化西番莲果皮中可溶性膳食纤维的酸酶法提取工艺  

Optimization of Acid Enzymatic Extraction Process of Souble Dietary Fiber from Passiflora edulis Peel by Response Surface Methodology

作　　者：蒋琳兰[1] 张丰进[1,2] 陈良云[1] 陈龙浩[1] 晏聪慧[1] 

机构地区：[1]广州军区广州总医院,广州510010 [2]华南理工大学生物科学与工程学院,广州510640

出　　处：《中国实验方剂学杂志》2015年第9期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东医院制剂研究开发技术平台建设项目(2011A08030004)

摘　　要：目的:优选西番莲果皮中可溶性膳食纤维的提取工艺,为其功能食品的研发提供参考。方法:在单因素试验基础上,选择柠檬酸溶液质量分数、料液比、耐高温α-淀粉酶添加量、复合酶反应温度为自变量,西番莲果皮可溶性膳食纤维提取量为因变量,利用Box-Behnken响应面法优选提取工艺参数。结果:最佳工艺条件为料液比1∶40,耐高温α-淀粉酶添加量5.0 U·g-1,柠檬酸溶液质量分数0.18%,复合酶反应温度80℃。可溶性膳食纤维提取率13.82%,与预测值的相对偏差-6.56%。结论:采用响应面法优化的西番莲果皮中可溶性膳食纤维提取条件稳定可靠,具有一定的实用价值。Objective： To optimize acid enzymatic extraction process of soluble dietary fiber from Passiflora edulispeel and provide references for research and development of its functional food. Method ： Based on single factor tests, with the concentration of citric acid solution, solid-liquid ratio, thermostable alpha amylase dosage, reaction temperature of complex enzyme as independent variables, extracting amount of soluble dietary fiber as dependent variable, Box-Behnken response surface methodology was used to optimize extraction conditions. Result： Optimum process conditions were as follows： solid-liquid ratio of 1：40, thermostable alpha amylase dosage of 5.0 U·g^-1, the concentration of citric acid solution of 0. 18% and reaction temperature of complex enzyme at 80 ℃. Yield of soubte dietary fiber was 13.82% , whose relative error was - 6.56% by comparing with the predictive value. Conclusion： Optimization of extraction process of souble dietary fiber from P. edulis peel by response surface methodology is accurate and reliable with some practical value.

关 键 词：西番莲 可溶性膳食纤维 酸酶法 耐高温Α-淀粉酶 木瓜蛋白酶 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]