新疆野生苹果(Malus sieversii)的总多酚、总黄酮提取物的抗氧化活性研究  

Antioxidant activity of polyphenolics and flavonoid extracted from wild apple(Malus sieversii)

作　　者：陈志娜[1] 师俊玲[2] 王继勋[3] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]西北工业大学生命科学学院,陕西西安710072 [3]新疆农业科学院园艺作物研究所,新疆乌鲁木齐830091

出　　处：《食品工业科技》2015年第10期143-147,152,共6页Science and Technology of Food Industry

基　　金：公益性行业(农业)研究专项(201003021)

摘　　要：以采集于新疆不同地区的20个野生苹果品种为研究对象,福林酚法测总多酚,分光光度计法测定总黄酮,DPPH、ABTS、还原能力法测定总抗氧化能力,相关系数法分析各个指标之间的相关性。结果显示,不同品种的野生苹果的总多酚、总黄酮及抗氧化活性之间具有显著差异,其中,HDM-23的总多酚含量最高(4714.33μmol没食子酸/100g鲜果),XY-31的总黄酮含量最高(2452.84mg芦丁/100g鲜果)。总多酚、总黄酮及抗氧化活性之间呈极显著(p<0.01)正相关,表明总多酚与总黄酮是野生苹果抗氧化活性的主要贡献物质。Twenty different lines of wild apple（Malus sieversii） collected from different places in Xinjiang,China were used as research objects. Total total polyphenol content was measured using the Folin-Ciocalteau method. Total flavonoid content was measured by spectrophotometer. Total antioxidant capacities were analyzed by ABTS assay, DPPH assay, and iron （ III ） to iron （ II ） reducing power assay, and the correlation between the various indicators was measured with Pearson correlation coefficient. It was found that HDM-23 had the highest total polyphenol content（4714.33pmol gallic acid/100g fresh matter）,and XY-31contained the highest amount of total flavonoid content（2452.84mg rutin/100g fresh matter）. The present study implied that total polyphenols contribute to the antioxidant properties of the wild apple（p〈0.01 ） and total flavonoid was an important phenolic compound in the wild apple varieties investigated.

关 键 词：野生苹果(Malus sieversii) 多酚 黄酮 抗氧化能力 

分 类 号：TS201.1[轻工技术与工程—食品科学]