肠炎沙门氏菌活菌标准物质的研制  

The Reference Materials of Preparation about Salmonella Enteritidis

作　　者：柯璐[1,2] 林杰[1,2] 戴晓丽[1,2] 黄嫦娇[1,2] 黄晓蓉[1,2] 

机构地区：[1]福建出入境检验检疫局技术中心,福建福州350003 [2]福建省检验检疫技术研究重点实验室,福建福州350003

出　　处：《食品研究与开发》2015年第5期111-115,共5页Food Research and Development

基　　金：福建省科技厅重点项目(2011Y01010219)

摘　　要：本文以肠炎沙门氏菌为选制菌种,用鸡肉糜混合保护剂配方作为活菌标准物质基质,采用冷冻真空干燥法研制活菌标准物质,共进行了3个批次的研究,每个批次分别制备100份熟制基质和100份生制基质的活菌标准物质。均匀性试验结果表明,生制基质和熟制基质的相对重复性标准差为2.185%和3.596%、相对再现性标准差为2.185%和3.514%,方差分析:P值均大于0.05,表明批次内以及批次间无显著差异。稳定性试验表明,在-20℃条件下,2种活菌标准物质稳定性良好。制备的肠炎沙门氏菌活菌标准物质具有相应的菌种特性,良好的稳定性,对研究同类型标准物质具有一定的参考价值,填补实验室检测质量控制对照物以及标准物质的空缺。Choose Salmonella enteritidis to the target species , take Chicken mince mixed protectant formula as matrix in Living Bacterium Reference Material , adopt Lyophilization method to develop Living Bacterium Reference Material, for the study of three batches total, each batch prepared the RM containing 100 bottles of Cooked Chicken Matrix and 100 bottles of Raw Chicken Matrix.The results of the uniformity experiment showed that, the repeatability standard deviation of raw chicken matrix and cooked chicken matrix is 2.185%and 3.596%, the reproducibility standard deviation is 2.185%and 3.514%, the results of the Analysis of Variance are P〉0.05, it indicated that there was no significant difference between the six batches of RM. Stability experiments showed that the RM stord at -20 ℃, the two kind of RM have good Stability. Conclusion：The Salmonella Enteritidis Living Bacterium Reference Material with corresponding strain properties, good stability, and them have great reference value to study the same type Reference Material , and fill the laboratory testing of quality control contrast and Reference Materials.

关 键 词：肠炎沙门氏菌 标准物质 鸡肉基质 

分 类 号：TS201.3[轻工技术与工程—食品科学]