甘肃河西走廊产区酿酒葡萄附生微生物区系分析  

Epiphytic microflora of wine grapes in Hexi corridor of Gansu

作　　者：王守一[1] 贠建民[1] 韩建平[1] 赵洪源[1] 邵晓庆[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2015年第2期77-83,共7页Journal of Gansu Agricultural University

基　　金：甘肃农业大学研究生导师基金项目(GAU-QNDS-201205)

摘　　要：以甘肃河西走廊具有代表性的3个产地（武威、张掖、嘉峪关）的4个品种（‘赤霞珠’‘品丽珠’‘蛇龙珠’‘黑比诺’）成熟期酿酒葡萄为研究对象,通过对葡萄浆果表面附生微生物的筛查、分离纯化与鉴定,分析葡萄附生微生物区系组成及其种群.结果显示：河西走廊产区酿酒葡萄附生菌相由11株细菌、10株酵母和14株霉菌组成.3个产地成熟期酿酒葡萄附生酵母菌的数量最大,达10^4～10^6cfu/g,附生细菌数量为10^4～10^5cfu/g,附生霉菌的数量为10^2～10^4cfu/g.酵母、霉菌、细菌数量受地域因素影响显著,但受葡萄品种因素影响不显著,且分离鉴定出灰葡萄孢霉（Botrytis cinerea）、交链孢霉（Alternaria alternata）和青霉属（Penicilliumspp.）等主要病害菌.研究结果可为原料采收后的前处理阶段开展有害微生物控制及评价原料耐贮性提供理论依据,并为酿造葡萄酒优良菌株选育提供种质资源.Four cultivars ripen wine grapes,Cabernet Sauvignon,Cabernet franc,Cabernet Gernischt and Pinot noir,were chosen from three different production areas（Wuwei,Zhangye and Jiayuguan）in Hexi Corridor of Gansu in order to determine their epiphytic microbial flora and the microbe species.The results showed that the epiphytic microflora was composed of eleven strains of bacteria,ten strains of yeasts and fourteen strains of moulds.For the three production areas,the yeast colony count was the most,which reached 10^4～10^6cfu/g,the bacteria colony count reached 10^4～10^5cfu/g,and the mould colony count reached 10^2～10^4cfu/g.The original had a significant effect on the epiphytic microbial colony count,including yeast,mould and bacteria,but the cultivars had no significant effect on the epiphytic yeast,mould and bacteria colony count.The pathogens of berries were also isolated and identified as Botrytis cinerea,Alternaria alternata,Penicilliumspp.

关 键 词：酿酒葡萄 附生微生物区系 河西走廊 

分 类 号：TS261.1[轻工技术与工程—发酵工程]