壳聚糖及其衍生物抗氧化活性的研究  

Antioxidant activity of chitosan and its derivatives

作　　者：刘欢[1] 夏光辉[1] 何文兵[1] 朱玲玲[1] 

机构地区：[1]通化师范学院长白山食品工程研究中心通化师范学院制药与食品科学学院,通化134000

出　　处：《食品科技》2015年第5期274-278,共5页Food Science and Technology

基　　金：吉林省科技发展计划项目(20130522093JH;20150101117JC)

摘　　要：以壳聚糖、羧甲基壳聚糖和盐酸壳聚糖为材料,研究3种壳聚糖及其衍生物对DPPH自由基、超氧阴离子自由基和羟自由基的清除能力以及还原能力和抗脂质氧化能力,并通化曲线拟合方法明确壳聚糖及其衍生物的IC50值。结果表明,随着浓度的增加,壳聚糖及其衍生物的抗氧化活性逐渐增强。3种壳聚糖及其衍生物中壳聚糖的抗氧化活性最强,其对DPPH自由基、超氧阴离子自由基和羟自由基的半清除率IC50值分别为0.91、4.87、0.28 mg/m L。The antioxidant activity of chitosan, carboxymethyl chitosan and hydrochloride chitosan were studied. Three chitosan and its derivatives were measured the scavenging effect of DPPH radical, superoxide radical, hydroxyl radical, reducing ability and anti-peroxidation ability. The IC50 values of the chitosan and its derivatives were defined through curvet fitting method. The results indicated that the antioxidant activity of the chitosan and its derivatives were increased gradually with the increase of concentration. Chitosan was the strongest than others. Its IC50 values for the half scavenging effect of DPPH radical, superoxide radical and hydroxyl radical were 0.91, 4.87 and 0.28 mg/m L.

关 键 词：壳聚糖 羧甲基壳聚糖 盐酸壳聚糖 抗氧化活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]