蒸发光散射-亲水作用色谱法测定味精和鸡精中的谷氨酸钠  

Determination of sodium glutamate in monosodium glutamate and chicken essence by evaporative light-scattering detector-hydrophilic interaction liquid chromatography

作　　者：汪辉[1,2] 徐睿鑫 李晰晖[1] 胡丽俐[1] 彭新凯[1,2] 夏立新[1,2] 

机构地区：[1]长沙市食品质量安全监督检测中心,湖南长沙410013 [2]湖南省食品安全生产工程技术研究中心,湖南长沙410013

出　　处：《中国食品卫生杂志》2015年第3期262-264,共3页Chinese Journal of Food Hygiene

基　　金：质检公益性行业科研专项项目(201310130)

摘　　要：目的建立了蒸发光散射-亲水作用色谱法测定味精和鸡精中的谷氨酸钠。方法色谱柱为Venusil HILIC（4.6 mm×250 mm,5μm）;柱温30℃;流动相为0.02 mol/L醋酸铵（冰醋酸调p H=3.5）-乙腈（80∶20,V/V）;流速1.5 ml/min;检测器为蒸发光散射检测器（ELSD）;样品经水超声提取,提取液过滤后供仪器分析。结果谷氨酸钠在6.0~20 g/L浓度范围内线性良好,味精和鸡精的平均回收率分别为98.5%和94.7%,RSD分别为1.07%和2.14%（n=5）。结论该方法适用于味精和鸡精中的谷氨酸钠的测定。Objective A evaporative light-scattering detector （ELSD） -hydrophilic interaction liquid chromatographic method was developed for determination of sodium glutamate in monosodium glutamate and chicken essence. Methods A Venusil HILIC （4.6 mm × 250 mm, 5 μm） column at 30 ℃ was used to separate the target compound. 0.02 mol/L ammonium acetate （glacial acetic acid adjust pH = 4. 5） -acetonitrile （20： 80, V/V） was used as mobile phase with the flow rate of 1.5 ml/min. The target compound was detected by ELSD. Water was used for ultrasonic extracting sodium glutamate in samples. The solution was analyzed by HPLC after filtration. Results Sodium glutamate showed good linearity with the detection range of 6.0-20 g/L. The spiked recoveries into monosodium glutamate and chicken essence were respectively 98.5% and 94.7% and relative standard deviations （RSDs） were respectively 1.07% and 2. 14% （n =5）. Conclusion The method could be used for determination of sodium glutamate in monosodium glutamate and chicken essence.

关 键 词：蒸发光散射检测器 亲水作用色谱 味精 鸡精 谷氨酸钠 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学]