超高压液相色谱-串联质谱法检测动物组织中26种β-受体激动剂  

Determination of 26 β-agonists in liver of pigs and ducks using ultra pressure liquid chromatography tandem quadrupole mass spectrometry

作　　者：刘先军[1] 王一红[1] 李帮锐[1] 丁力[1] 冯家力[1] 陈东洋[1] 

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410003

出　　处：《中国食品卫生杂志》2015年第3期265-270,共6页Chinese Journal of Food Hygiene

摘　　要：目的建立超高压液相色谱-串联四极杆质谱（UPLC-MS/MS）同时测定猪肝、鸭肝中26种β-受体激动剂的检测方法。方法样品经三氯乙酸溶液提取,低温离心后,上清液用MCX固相萃取柱净化,Waters ACQUITY UPLCTMBEH C18色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,UPLC-MS/MS进行测定,基质加标标准曲线法定量。结果该方法的平均回收率为76.4%~115.4%,RSD〈15.0%,方法的定量限为0.1~0.7μg/kg。结论该方法操作简单、灵敏度高、重现性良好,适用于动物性食品中β-受体激动剂的快速检测。Objective To develop an analytical method for the determination of 26 β-agonists in animal liver tissue based on ultra pressure liquid chromatography tandem mass spectrometry （UPLC-MS/MS）. Methods Pig liver and duck liver were extracted using trichloracetic acid solution. Then the extract were purified and enriched by Oasis MCX solid phase extraction cartridges. Target compounds were separated on a Waters ACQUITY UPLCTM BEH C18 column with gradient elution using methanol and water containing 0. 1% formic acid. Quantification was performed with matrix-fortified standard calibration. Results Average recoveries of 26 β-agonists spiked at three concentrations ranged from 76.4% to 115.4% with relative standard deviation lower than 15.0%. The limits of quantitation （LOQs） for the method ranged from 0. 1 to 0. 7 μg/kg. Conclusion Due to its high sensitivity and good reproducibility, the method is suitable for the determination of target agnoists in animal liver tissue.

关 键 词：超高压液相色谱-串联质谱法 固相萃取 β-受体激动剂 肝脏 兽药残留 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.63[医药卫生—公共卫生与预防医学]