新鲜槐花精油化学成分的GC-MS分析及其抗氧化活性  

GC-MS Analysis of the Chemical Constituents and Antioxidant Activity of Essential Oil from Fresh Sophora japonica L.

作　　者：朱广琪 陈菲[1] 冯宇 王静宇[1] 任慧芳[1] 马雪梅[1] 毕淑峰[1] 

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245041

出　　处：《中国调味品》2015年第6期115-118,共4页China Condiment

基　　金：国家自然科学基金(21302004);安徽省大学生创新创业计划项目(AH201310375043)

摘　　要：采用水蒸气蒸馏法提取新鲜槐花精油,利用气相色谱-质谱联用技术分析其化学成分;以DPPH和ABTS自由基的清除作用评价精油的抗氧化活性。从新鲜槐花精油中共鉴定出55种化合物,占精油总量的81.82%,主要成分有正己醇(11.12%)、芳樟醇(8.50%)、棕榈酸(6.12%)、反式-橙花叔醇(5.81%)、6,10,14-三甲基-2-十五烷酮(5.54%)。精油对DPPH,ABTS自由基有明显的清除作用,且样品量与清除率呈量效关系。精油对DPPH,ABTS自由基的清除率为50%时,精油体积分别为55.83,15.81μL。新鲜槐花精油以醇、萜类及其含氧衍生物、醛为主,含有多种生物活性成分,具有较好的抗氧化活性。The essential oil from fresh Sophora j aponica L.is extracted by water-steam distillation and its chemical constituents are analyzed by GC-MS.The antioxidant activity of essential oil is evaluated by scavenging capacity against DPPH＆#183; and ABTS＆#183;.Fifty-five compounds,which occupy 81.82% the of total constituents,are identified from the essential oil.The major constituents are n-hexyl alcohol （1 1.12%）,linalool （8.50%）,palmitic acid （6.12%）,trans-nerolidol （5.81%）,and 6,10, 14-trimethyl-2-pentadecanone （5.54%）.The essential oil exhibits significant scavenging capacity against DPPH ＆#183; and ABTS ＆#183; in a concentration-dependent fashion.When the scavenging rate against DPPH ＆#183; and ABTS ＆#183; of essential oil is 50%,the volume of essential oil is 55.83, 15.81 μL respectively.The essential oil,which contains many bioactive constituents,is mainly composed of alcohols,terpenes and their derivatives and aldehydes,and shows significant antioxidant activity.

关 键 词：新鲜槐花 挥发油 气相色谱-质谱联用 化学成分 抗氧化活性 

分 类 号：TS264.3[轻工技术与工程—发酵工程]