ATP生物发光法快速测定物体表面的菌落总数  

Rapid determination of aerobic plate count on solid surfaces by ATP bioluminescent method

作　　者：侯玉柱[1] 田雨[1] 柯润辉[1] 尹建军[1] 宋全厚[1] 

机构地区：[1]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2015年第2期217-220,共4页Food and Fermentation Industries

基　　金："十二五"国家科技支撑计划(2012BAD29B04):食品加工过程质量安全在线监测与控制技术研究示范

摘　　要：以大肠埃希氏菌作为指示菌考量了ATP生物发光法测定物体表面卫生状况的准确性,并在实验室内模拟食品企业卫生管控,采用ATP生物发光法对具有代表性的台面进行细菌总数测定,与国家标准微生物检测方法进行了比对分析。结果显示:大肠埃希氏菌的ATP荧光值与菌落总数的线性相关性良好,相关系数R2为0.982 6;以实验室内指定台面作为盲样进行对比测定,其ATP荧光值与菌落总数的线性相关性良好,相关系数R2达到0.915 9,说明ATP生物发光法能够准确评估和测定物体表面的卫生状况。In this paper Escherichia coli was used as an indicator to evaluate the surface hygiene by ATP bioluminescent method. We also simulated hygienic control from food factory to laboratory and determined the aerobic plate count of representative surface in laboratory by this method. Meanwhile, it was analyzed and compared with national standard microbiological examination. The results showed a good linear relationship between ATP fluorescence values and aerobic plate counts of E. coli, and their correlation coefficient R^2 was 0. 982 6. The designated laboratory surfaces were taken as blind samples. There was a good linear relationship between their ATP fluorescence values and aerobic plate counts, and their correlation coefficient R^2 was 0. 915 9. It illustrated that ATP bioluminescent method could assessed and determined accurately solid surface hygiene.

关 键 词：ATP生物发光法 菌落总数 数学模型 预测 

分 类 号：O657.3[理学—分析化学] TS207.4[理学—化学]