基于核酸适体微球的沙门氏菌检测方法研究  

Study on detection method of salmonella using aptamer- conjugated microbeads

作　　者：金娜 孔先利 林敏 左鹏[3] 

机构地区：[1]温州出入境检验检疫局,浙江温州325000 [2]台州出入境检验检疫局,浙江台州318000 [3]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《食品工业科技》2015年第13期321-324,333,共5页Science and Technology of Food Industry

摘　　要：目的:研究基于核酸适体的微球芯片系统应用于沙门氏菌检测的可行性。方法:采用偶联有沙门氏菌适体的微球作为载体,以荧光作为检测信号建立微球芯片体系,并优化影响检测结果的各项因素。结果:通过此方法检测20份水产样品,6份检出沙门氏菌,检出率为30%。结论:建立的检测体系能实现对沙门氏菌的快速,准确的检测,且具有较高的特异性。Objective. To investigate the feasibility of aptamer- conjugated microbeads for the detection of salmonella.Methods.Amino-terminal aptamer probes were covalently attached onto the surface of microbeads. Fluorescence from microbeads was detected as signal source, and parameters were optimized which may affect detection of the target.Results=Salmonella were detected in six of 20 aquatic product samples by this method,and the detection rate was 30%.Conclusion：The aptamer-conjugated microbeads can be used for high throughput and accurate detection of salmonella.

关 键 词：沙门氏菌 适体 微球 检测 

分 类 号：TS201.1[轻工技术与工程—食品科学]