琼脂酶产生菌发酵产酶条件优化  

Optimization fermentation conditions of agarase-producing bacteria

作　　者：朱英莲[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,青岛266109

出　　处：《食品科技》2015年第6期11-13,共3页Food Science and Technology

基　　金：国家教育部高等学校特色专业建设项目(TS10943);山东省高等教育名校建设工程项目(XJG2013088;XJP2013043)

摘　　要：琼脂酶是一种研究海藻遗传工程的工具酶,对一株产琼脂酶菌的产酶条件进行了优化,研究不同蛋白胨浓度、酵母膏浓度、装液量、接种量对该菌产酶的影响。通过正交试验得出该菌的最优产酶条件为蛋白胨浓度7 g/L,酵母粉浓度1.25 g/L,装液量150 m L,接种量10%。Agarase enzyme is a kind of tool enzyme for genetic engineering of algae. In this paper, the optimum fermentation conditions of an agarase-producing bacteria were researched. The different conditions of peptone concentration, yeast extract concentration, volume of medium, inoculability quantity on agarase-producing effect were studied. Results were concluded that the optimum fermentation conditions were as follows： peptone concentration was 7 g/L, yeast concentration was 1.25 g/L, volume of medium was 150 mL and inoculability quantity was 10%.

关 键 词：琼脂酶 酶活力 发酵 正交试验 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]