油莎豆复合酶法抗性淀粉的制备及纯化工艺  

Preparation and purification process of cyperus esculeutus resistant starch by compound enzymes

作　　者：刘雷[1] 邹险峰[1] 雷海容[1] 

机构地区：[1]长春大学农产品深加工吉林省重点实验室,长春130022

出　　处：《食品科技》2015年第6期296-299,共4页Food Science and Technology

摘　　要：以油莎豆淀粉为原料,通过高温α-淀粉酶水解,普鲁兰酶脱支,对淀粉进行连续处理,研究复合酶法油莎豆抗性淀粉的制备及纯化工艺条件,单因素及正交实验结果表明,油莎豆淀粉双酶法制备抗性淀粉酶解的最佳工艺条件是:高温α-淀粉酶添加量2μ/g、酶解温度95℃、酶解时间15 min。普鲁兰酶添加量15μ/g、酶解温度50℃、酶解时间20 h。此条件下制备的油莎豆抗性淀粉得率为39.48%,抗性淀粉含量为57.72%。With Cyperus esculeutus starch as material, the preparation and purification conditions of enzymolysis on Cyperus esculentus starch by thermostable α-amylase and pullulanase were studied through single factor and orthogonal experiments. The optimal process parameters were followed： 2 μ/g of addition quantity, 95 ℃ of hydrolysis temperature, 15 rain of hydrolysis time for thermostable α-amylase; 15μ/g of addition quantity, 50 ℃; of hydrolysis temperature, 20 h of hydrolysis time for pullulanase. Under the above conditions, the rate of Cyperus esculentus resistant starch was 39.48%, and the content of resistant starch was 57.72%.

关 键 词：油莎豆 抗性淀粉 高温Α-淀粉酶 普鲁兰酶 

分 类 号：TS234[轻工技术与工程—粮食、油脂及植物蛋白工程]