反相高效液相色谱梯度洗脱法同时检测饮料中9种食品添加剂  

Simultaneous determination of nine food additives in drinks by reversed-phase high performance liquid chromatography with gradient program

作　　者：杨毅华 杨笑 陈波[2] 

机构地区：[1]浙江省宁波市北仑区食品安全检测中心,宁波315800 [2]浙江省宁波市北仑区疾病预防控制中心,宁波315800

出　　处：《中国食品添加剂》2015年第6期188-193,共6页China Food Additives

摘　　要：目的：建立反相高效液相色谱法同时测定饮料中9种食品添加剂的分析方法。方法：样品经处理后，在ZORBAXSB—C18（4．6mm×250ram×51ma）色谱柱上，以甲醇-三氟乙酸-醋酸铵作为流动相，流速1mL／min，柱温40℃。采用梯度洗脱分离，紫外检测器变波长检测，外标法定量。结果：安赛蜜、柠檬黄、苯甲酸、糖精钠、山梨酸、苋菜红、胭脂红、日落黄、亮蓝在1．0～50mg／L范围内具有良好的线性，9种待测物的回收率在91．0％～103．3％，RSD均小于5．0%，最低定量检出限为0．10mg／kg。结论本方法简便、灵敏、重现性好，能满足实际工作的需要。Objective ： To develop a method for the simultaneous determination of 9 food additives by reversedphase high performance liquid chromatography. Methods ： After extracted, samples were separated in a ZORBAX SB-C18 （4.6mm ×250mm × 5μm） column with gradient program by using methylalcohol/trifluoroacetic acid/ ammonium acetate as mobile phase.Detection was performed with UV detector of variable wave-length and the column temperature was hold at 40℃.Quantitative analysis was carried out by measuring peak area and comparing it with external standard.Results ： Good linearly in the range of 1.0-50mg/L for acesulfame, citron yellow, benzoic acid, saccharin, sorbicacid, amaranth, ponceau, sunset yellow, brilliant blue.Recoveries were in the range of 91.0- 103.3%, and precision values expressed as relative standard deviation （RSDs） were lower than 5.0%.The limits of quantification （LOQs） in samples were in the range of O.lOmg/kg.Conclusion. This method is simple, sensitive and reproducible, which can be applied to the routine analyses.

关 键 词：高效液相色谱法 食品添加剂 防腐剂 甜味剂 色素 

分 类 号：TS202.3[轻工技术与工程—食品科学]