固定化酸性蛋白酶载体的选择及其性质  

Carrier Screening for and Properties of Immobilized Acid Protease

作　　者：王冕[1] 王如福[1] 张茜[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《食品科学》2015年第13期89-94,共6页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2011AA10090401);山西省科技攻关项目(20110311042-3;20130311030-2)

摘　　要：选用海藻酸钠(sodium alginate,SA)分别与3种天然高分子材料制作复合凝胶载体,以酶活回收率为评价指标,采用单因素试验和正交试验确定最佳载体材料及最佳固定化条件。结果表明,SA-黄原胶复合凝胶为最佳载体,其最佳工艺条件为:SA与黄原胶质量比例为6∶1,Ca Cl2质量浓度为5.0 g/100mL,固定化时间为2.5 h,在此条件下固定化酶活回收率为74.12%。对固定化酸性蛋白酶酶学性质进行研究,结果表明,其最适p H值为3.0、最适反应温度为45℃,均与游离酶相同,热稳定性优于游离酶,动力学参数Km值高于游离酶,操作半衰期为17.28d。The activity of acid protease immobilized in different composite gel carriers made from sodium alginate（SA）combined separately with three natural polymers was measured to select the best immobilization carrier and conditions using single factor experiments and orthogonal array design.The results showed that the best carrier was composed of SA and xanthan gum and the best immobilization conditions were determined as follows：CaCl_2 concentration,5.0 g/100 mL,SA to xanthan gum ratio,6：1,and immobilization time,2.5 h.Experiments conducted under these conditions gave an activity recovery of 74.12%.The enzymatic properties of immobilized protease indicated there was no variation in optimum pH and optimum temperature when compared with the free enzyme.Compared with the free enzyme,thermal stability of immobilized acid protease was enhanced,and the kinetic parameter K_m was improved.The half-life of the immobilized protease was 17.28 d.

关 键 词：酸性蛋白酶 固定化酶 载体 回收率 酶学性质 

分 类 号：Q814[生物学—生物工程]