三斑海马蛋白肽ACE抑制活性的研究  

Angiotensin I- converting enzyme( ACE) inhibitory activity of enzymatic hydrolysate from three- spot hippocampus

作　　者：徐克寒[1] 申铉日[1] 陈国华[2] 

机构地区：[1]海南大学食品学院,海南海口570228 [2]海南大学海洋学院,海南海口570228

出　　处：《食品工业科技》2015年第15期96-99,共4页Science and Technology of Food Industry

基　　金：十二五国家科技支撑计划课题(2013BAB01B04)

摘　　要：本文通过三斑海马酶解液的制备、ACE抑制活性肽的分离和体外消化模型的建立研究了三斑海马蛋白肽的ACE抑制活性。利用碱性蛋白酶制备得到具有ACE抑制活性的三斑海马酶解液,经葡聚糖凝胶层析分离纯化后,得到具有较高ACE抑制活性的组分(其IC50为0.91 mg/m L);通过对该组分的氨基酸组成和体外消化模型分析,发现该组分中疏水性氨基酸的含量为50.48%,消化酶作用后,显著提高ACE抑制活性,经判断,该组分中的蛋白肽为前体型抑制剂。In this paper, the ACE-inhibitory activity of three-spot hippocampus peptides were evaluated by studying the preparation technique of three-spot hippocampus enzymatic hydrolysate, screening chromatography separation of ACE-inhibitory peptides and establishing the model.The enzymatic hydrolysate with ACE-inhibitory activity was prepared by alkaline protease,and then separated and purified by gel filtration chromatography.After determination,the lower molecular fraction exerted the highest ACE-inhibitory activity（ IC50 was 0.91 mg/mL）. The composition of amino acids in it was analyzed,and the test result indicated that the content of hydrophobic amino acids was 50.48%. In addition, it was suggested that the ACE-inhibitory peptides acted as ＇ prodrug-type＇ inhibitor after establishing the model of artificial gastrointestinal digestion.ACE- inhibitory activity was increased significantly under condition of digestive enzyme digestion.

关 键 词：三斑海马 ACE抑制 酶解液 

分 类 号：TS201.2[轻工技术与工程—食品科学]