谢氏丙酸杆菌与不同乳杆菌混合培养  

Co-culture of Propionibacterium shermanii with different Lactobacillus spp.

作　　者：曾贤慧[1] 雷柳琳 庄旭昇[1] 金李玲[1] 庞旭 朱剑锋 胡文锋[1] 

机构地区：[1]华南农业大学食品学院,广州510642 [2]生物源生物技术(深圳)有限公司,深圳518055

出　　处：《食品科技》2015年第7期23-31,共9页Food Science and Technology

基　　金：深圳市南山区科技创新局创新研发项目(KC2014JSCX0037A);生物源生物技术(深圳)有限公司科技专项(生物源研字2013003)

摘　　要：为了开发多菌微生态制剂,可通过混合发酵加以实现。该方法不但一次发酵得2种或以上益生菌细胞,又能获得对致病菌有抑杀作用的代谢产物,从而大大降低发酵成本。以谢氏丙酸菌与不同乳杆菌进行混合发酵,以大肠杆菌和金黄色葡萄球菌为指示菌,通过牛津杯法筛选,高效液相法测定其有机酸含量。得到:谢氏丙酸菌与鼠李糖乳杆菌6003混合培养抑菌效果最佳,乳酸、丙酸产量分别达到17.65、28.55 g/kg(湿重);对2种指示菌的抑菌圈直径分别为17.63、19.39 mm;之后结合单因素实验和响应面法优化,获得最佳培养条件:葡萄糖0.21 mol/L,复配氮源添加量2.17%,初始p H6.72,K2HPO4·H2O添加量为0.52×10-3 mol/L,MnSO4·H2O添加量为0.59×10-5 mol/L,优化后混合培养液对2种指示菌平均抑菌直径分别为18.49、22.63 mm,比优化前提高4.93%、16.72%。乳酸、丙酸产量为43.03、16.92 g/kg(湿重)。因此,谢氏丙酸菌与鼠李糖乳杆菌6003混合培养能提高产物抑菌效果,是一种值得开发的生产微生态制剂的新方法。The aim of this research is to get a new probiotics products by the co-culture of Propionibacterium shermanii and different lactic acid bacteria.The co-culture process can get two or more kinds of probiotics and metabolites with the role of suppression and killing the pathogens at the same time,thus reducing the cost.Using Propionibacterium shermanii and different Lactobacillius spp.as the original strains,the antibacterial effect of metabolites on Escherichia coli and Staphylococcus aureus were analysis by Oxford cup and the production of organic acids was measured by HPLC.The metabolites of Propionibacterium shermanii and Lactobacillus rhamnosus 6003 co-culture showed the best antibacterial effect.The inhibition zones of two strains were 17.63 mm and 19.39 mm.The production of lactic acid and propionic acid were 17.65 g/kg and 28.55 g/kg（wet weight） respectively.Then the single factor and the response surface analysis were conducted to improve the antibacterial effect.The optimum medium formula was 0.21 mol/L glucose,2.17% mixed nitrogen source and the initial pH 6.72,0.52×10-（-3） mol/L K_2HPO_4·H_2O,0.59×10-（-5） mol/L MnSO_4·H_2O.The average diameter of inhibition zone of the two strains were up to 18.49 mm and 22.63 mm,4.93% and 16.72% higher,compared with the co-culture first screening.The production of lactic acid and propionic acid were 43.03 g/kg and 16.92 g/kg（wet weight）.Propionibacterium shermanii and Lactobacillus rhamnosus 6003 co-culture can improve the antibacterial effect and this co-culture will be an effective way to produce complex probiotics.

关 键 词：谢氏丙酸杆菌 鼠李糖乳杆菌 混合培养 抑菌圈 

分 类 号：TS201.3[轻工技术与工程—食品科学]