中国六省份零售整鸡中环丙沙星与头孢噻肟双耐药印第安纳沙门菌流行状况及分子分型研究  

Epidemic condition and molecular subtyping of ciprofloxacin and cefotaxime co-resistant Salmonella Indiana isolated from retail chicken carcasses in six provinces, China

作　　者：胡豫杰[1] 赫英英 王晔茹[1] 崔生辉[3] 陈秋霞[4] 刘桂华[5] 陈倩[6] 周刚[7] 杨保伟[8] 黄金林 于红霞[2] 李凤琴[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]山东大学公共卫生学院营养与食品卫生研究所 [3]中国食品药品检定研究院食品化妆品检定所 [4]广东省疾病预防控制中心病原微生物所 [5]吉林省疾病预防控制中心微生物所 [6]北京市疾病预防控制中心营养与食品卫生所 [7]内蒙古自治区食品药品监督管理局食品药品检验所 [8]西北农林科技大学食品与科学学院 [9]扬州大学江苏省人兽共患病重点实验室

出　　处：《中华预防医学杂志》2015年第8期716-721,共6页Chinese Journal of Preventive Medicine

基　　金：国家高技术研究发展计划（2012AA101603）

摘　　要：目的：对中国6个省份零售整鸡中对环丙沙星与头孢噻肟双耐药的沙门菌流行状况及分子分型进行研究。方法对我国6个省份市售整鸡样品中分离的2629株沙门菌进行耐药性实验，筛选出对环丙沙星和头孢噻肟双重耐药的菌株，进行血清分型、超广谱β-内酰胺酶（ESBLs）表型确证和PFGE遗传特性研究。结果对环丙沙星与头孢噻肟双耐药的沙门菌共计227株（8.52%，227/2629），北京、吉林、广东、江苏、陕西、内蒙的检出率分别为11.67％（99/874）、8.20％（60/726）、1.39％（7/502）、15.61％（42/260）、8.56％（16/186）、0（0/81）；224株为印第安纳沙门菌，其中213株（95.10%）为ESBLs阳性。所有双耐印第安纳沙门菌均耐5种以上的抗生素，17.86%的菌株（40/224）对除碳青霉烯类外的10种抗生素均耐药；50.89%的菌株（114/224）对9种抗生素耐药，25.45%的菌株（57/224）对8种抗生素耐药。224株双耐印第安纳沙门菌划分为32个基因簇和150种PFGE带型，双耐印第安纳沙门菌带型既具有地域差异，相同或不同的省份及采样时间均发现相同带型菌株。结论中国零售整鸡中环丙沙星与头孢噻肟双耐药沙门菌污染严重，是双耐药沙门菌的重要储存库，分子分型结果提示沙门菌存在交叉污染或共同污染来源。Objective To elucidate the epidemic condition and molecular subtyping of ciprofloxacin and cefotaxime co-resistant Salmonella Indiana（S. Indiana）isolated from retail chicken carcasses in six provinces of China. Methods A total of 2 647 Salmonella strains isolated from retail chicken carcasses collected from six provinces of China were subjected to antimicrobial susceptibility testing. All Salmonella isolates co-resistant to ciprofloxacin and cefotaxime were further characterized by serotyping, extended-spectrum beta-lactamases （ESBLs） producing strains screening and pulsed field gel electrophoresis （PFGE） typing. Results Among 2 629 Salmonella isolates tested, 227 （8.52%） isolates were co-resistant to ciprofloxacin and ceftazidime/cefotaxime （Beijing：11.67%（99/874）,Jilin：8.20%（60/726）, Guangdong： 1.39%（7/502）,Jiangsu： 15.61%（42/260）,Shaanxi： 8.56%（16/186）,Inner Mongolia： 0（0/81））, and 224 of them were identified as S. Indiana. 213（95.10%）isolates of S. Indiana were ESBLs＆amp;nbsp;producing strains. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates developed a multi-drug resistant profile and 17.86%（40/224）of them were resistant to all antibiotics tested except carbapenems, and 50.89%（114/224）of them resistant to 9 antibiotics, additionally, 25.45%（57/224）of them showed multi-drug resistance to 8 antibiotics. All ciprofloxacin and cefotaxime co-resistant S. Indiana isolates were divided into 32 PFGE clusters and 150 PFGE patterns. Strains of S. Indiana from same or different sampling site and time seemed to either share the same PFGE patterns or be differential to each other in different regions. Conclusion The results indicated that chicken carcasses collected from parts of China were heavily contaminated by ciprofloxacin and cefotaxime co-resistant S. Indiana and could serve as an important reservoir of ciprofloxacin and cefotaxime co-resistant Salmonella. Molecular subtyping results indicated that cross contamination or co

关 键 词：沙门菌属 环丙沙星 头孢噻肟 电泳 凝胶 脉冲场 耐药 

分 类 号：R155.5[医药卫生—营养与食品卫生学]