石墨炉原子吸收法测定高盐食品中的铅及其干扰消除的研究  

The determination of lead and interference elimination in high salty food by GFAAS

作　　者：胡曙光[1,2] 苏祖俭 蔡文华[1] 何健飞[3] 欧天成 黄振波[5] 黄伟雄[1] 梁旭霞[1] 张学武[1] 

机构地区：[1]广东省疾病预防控制中心国家食品安全风险监测重金属参比实验室,广东广州510300 [2]中山大学生命科学院,广东广州510275 [3]清远市疾病预防控制中心,广东清远526060 [4]肇庆市疾病预防控制中心,广东肇庆526060 [5]河源市疾病预防控制中心,广东河源517000

出　　处：《中国食品卫生杂志》2015年第4期394-398,共5页Chinese Journal of Food Hygiene

基　　金：广东省医学科研基金课题(A2013068);2014年食品安全国家标准整合项目(ZHENGHE-2014-347)

摘　　要：目的通过研究高盐食品中石墨炉原子吸收法测定铅的基体干扰模式,探讨各种基体改进剂、升温程序和校正模式对减少或消除氯化钠干扰的效果与能力,建立了石墨炉原子吸收法测定高盐食品中铅的方法。方法采用微波消解、湿法消解、高压罐消解和直接稀释法4种前处理方式,硝酸钯-磷酸二氢铵为混合基体改进剂,标准加入法测定高盐食品中铅含量。结果选择283.3 nm为测定波长,磷酸二氢铵-硝酸钯作为基体改进剂,标准加入法为校正模式,在盐度2.2%以下可消除氯化钠的基体干扰。该方法的线性范围为1.8~40.0μg/L,当称样量为0.5 g,定容量为10 ml时,定量限为0.036 mg/kg。结论在较高的盐度中,该方法可消除石墨炉原子吸收测定食品中铅的基体干扰,提高了分析结果的准确性和可靠性。本研究为国标的修订与整合做好了技术储备。Objective A method of determination of lead in high salty food by GFAAS was established, and the interference model, various matrix modifiers, temperature program and correction model were explored. Methods Lead in high salty food was measured by standard addition method with Pd （NO3 ）2-NH4H2PO4 as mixed matrix modifier after four kinds of pretreatment methods including microwave digestion, wet digestion, high-pressure tank digestion and direct dilution were adopted. Results While the salinity was below 2.2% , the interference could be eliminated by standard addition method with Pd （NO3）z-NH4HzPO4 at the wavelength of 283.3 nm. The linear range was 1.8-40 Ixg/L. When O. 5 g sample were diluted to 10 ml, the limit of quantification was 0. 036 mg/kg. Conclusion This method could eliminate the interference of high salinity during lead determination by GFAAS and improve the accuracy and reliability. The study t^rovided zood technical reserves for the revision and integration of national standards were established.

关 键 词：高盐食品 铅 石墨炉原子吸收法 痕量分析 食品污染物 食品安全 

分 类 号：R155.5[医药卫生—营养与食品卫生学] P618.42[医药卫生—公共卫生与预防医学]