非水相酶促合成L-抗坏血酸辛酸酯及其结构表征  

Lipase-catalyzed synthesis of L-ascorbyl octanoate in non-aqueous phase and its structural characterization

作　　者：张淑青[1] 潘丽军[1] 姜绍通[1] 操丽丽[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽省农产品精深加工重点实验室,合肥230009

出　　处：《中国油脂》2015年第8期59-64,共6页China Oils and Fats

基　　金：“十一五”国家科技支撑计划项目(2010BAD01B07);农业科技成果转化资金项目(2013GB2C300217)

摘　　要：以中链脂肪酸正辛酸为酰基供体,在有机相体系中利用脂肪酶催化酯化反应合成L-抗坏血酸辛酸酯。根据初步试验结果,选取对L-抗坏血酸酯化转化率影响显著的因素,进行响应面试验设计,建立回归模型,得到最优参数组合。结果表明:反应时间、反应温度、反应时间和反应温度的交互作用以及反应时间和底物浓度的交互作用对L-抗坏血酸酯化转化率均有显著影响(p<0.05),回归模型可用。L-抗坏血酸辛酸酯酶法合成最优工艺条件为反应时间11.45 h、底物(L-抗坏血酸)浓度0.24 mol/L、反应温度55.3℃、脂肪酶用量(以L-抗坏血酸质量计)19.18%,此条件下L-抗坏血酸酯化转化率可达83.31%。产物经IR、MS、1H NMR和13C NMR结构表征,证实正辛酸被引入到了L-抗坏血酸的第6位羟基上。L- ascorbyl octanoate was synthesized through lipase -catalyzed esterification in organic sys- tem with mid - chain fatty acid n - octanoic acid as acyl donor. The reaction parameters that highly influ- enced the conversion rate of L -ascorbic acid were selected as main variables to design the response sur- face experiment on the basis of preliminary experiments, then a regression model was established and the optimal synthesis conditions were obtained. The results indicated that reaction time, reaction tempera- ture, interaction between reaction time and reaction temperature and interaction between reaction time and substrate concentration had significant effects on the conversion rate of L - ascorbic acid （p 〈 0.05 ）, and the regression model could be used to predict the conversion rate of L - ascorbic acid. The optimal enzymatic synthesis conditions of L -ascorbyl octanoate were obtained as follows： reaction time 11.45 h, substrate （L - ascorbic acid） concentration 0.24 mol/L, reaction temperature 55.3℃, dosage of lipase （based on the mass of L -ascorbic acid） 19.18%. Under these conditions, the conversion rate of L -ascor- bic acid reached 83.31%. In addition, the product was characterized by IR, MS, 1H NMR and 13C NMR, and the result showed that n- octanoic acid was introduced into the sixth hydroxyl of L- ascorbic acid.

关 键 词：正辛酸 L-抗坏血酸 脂肪酶 L-抗坏血酸辛酸酯 结构表征 

分 类 号：TS202.3[轻工技术与工程—食品科学] O657.2[轻工技术与工程—食品科学与工程]