发酵荔枝渣制备微生物油脂  

Study on fermentation of litchi residues to produce microbial lipid

作　　者：邱松山[1] 王彦安[1] 林梦红 姜翠翠[1] 李春海[1] 

机构地区：[1]广东石油化工学院环境与生物工程学院果蔬加工与贮藏工程中心

出　　处：《食品与发酵工业》2015年第8期117-122,共6页Food and Fermentation Industries

基　　金：广东省教育部产学研结合项目(2013B090600032);茂名市科技计划项目(2014007);广东省科技计划项目(2014B040404027)

摘　　要：利用粘红酵母发酵荔枝渣水解液对制备微生物油脂,为综合利用荔枝加工下脚料提供参考。通过摇床培养确定粘红酵母种子液的最佳培养时间及最适装液量,通过单因素试验及正交试验优化发酵工艺参数,获得产油脂最优发酵条件,并对所得微生物油脂理化性质进行分析。结果表明:(1)粘红酵母种子液的最佳培养时间为24 h;发酵液最适装液量为40 m L/250 m L;最佳工艺条件为初始糖度8°Brix、初始p H值5.5、接种量10%、发酵时间96 h。在此基础上测得微生物平均油脂浓度、生物量和油脂含量分别为:0.378 g/L、15.670 g/L和2.414%,产脂能力为378.3 mg/L。(2)对得到的微生物油脂进行理化指标分析,所得微生物油脂呈黄色透明液体,无特殊气味,平均相对密度为1.104 4 g/m L,折光指数为1.459 0,酸价为5.39(mg/g),碘价为101.8(g I2/100 g),过氧化值为9.44(mmol/kg)。In this paper,litchi processing residues was fermented with the lipid-producing rhodotorula glutinis to produce microbial lipid. The optimal seeding time and volume of rhodotorula glutinis seed broth in flask were studied.Meanwhile,the process of microbial oil production was optimized by single factor experiment and orthogonal experiment to obtain the optimal fermentation conditions,and the physicochemical properties of the oil were analyzed. Results showed that the capacity of lipid production of 378. 3 mg / L was achieved under the optimal conditions as follows：24 h of seeding time,40 m L /250 of liquid volume in flask,8°Brix of initial sugar concentration,5. 5 of initial p H,10% of inoculum,and 96 h of fermentation time. Meanwhile,the biomass reached 15. 670 g / L and the oil content was 2. 414%. The microbial oil was yellow transparent liquid with average relative density of 1. 104 4 g / L and refractive index of 1. 459 0. Its acid value was 5. 39（ mg / g）,the iodine value was 101. 8（ g I2/100 g）,and the peroxide value was 9. 44（ mmol / kg）. The production of microbial oil with litchi residue reached the dual purpose of waste utilization and energy development and had the broad application prospect.

关 键 词：微生物油脂 发酵 荔枝渣 

分 类 号：TQ645[化学工程—精细化工] TQ920.6[轻工技术与工程—发酵工程]