双酶复合水解蜂蛹蛋白工艺研究  

Research on Hydrolysis of Double-enzyme Complex Process of Defatted Honeybee Pupae Protein

作　　者：卢红冲 吴平[1] 陈迪想 钟方旭[1] 陈西喆 鄢又玉 

机构地区：[1]武汉轻工大学生物与制药工程学院,武汉430023 [2]湖北神农蜂语生物产业有限公司,十堰442000

出　　处：《食品工业》2015年第9期24-28,共5页The Food Industry

摘　　要：研究了脱脂蜂蛹蛋白的酶法水解工艺。以水解度和多肽得率为指标,从中性蛋白酶、碱性蛋白酶、木瓜蛋白酶、菠萝蛋白酶、风味酶中选出中性蛋白酶和碱性蛋白酶进行双酶复合水解。采用单因素与响应面优化设计,得出最佳酶解工艺条件为:复合酶比例1︰1(g/g)、加酶量4.67%、p H 8.27、料液比1︰12(g/m L)、酶解温度48.44℃、酶解时间4 h。此优化试验条件下,蜂蛹蛋白理论水解度46.05%,实际水解度为45.89%。此工艺可推广应用于蜂蛹蛋白多肽产业化生产的备选工艺。Enzymatic hydrolysis technology of defatted honeybee pupae is studied. The degree of hydrolysis （DH） and the yield of polypeptide are taken as evaluation index, neutral and alkaline protease are selected out to be further studied from neutral protease alkaline protease, papain, bromelain and flavor enzyme. On the basis of single factor and response surface experiments, the optimum enzymatic condition is obtained： the mass ratio of complex enzyme 1 ： 1 （g/g）, enzyme amount 4.67%, pH 8.27, the ratio of material and solvent 1 ： 12 （g/mL）, enzymatic temperature 48.44 ~C and reaction time is 4 h. Under such optimized conditions, the theoretical DH of honeybee pupae protein is 46.05% and the actual one is 45.89%. Both of which are fit well and the optimum enzymatic technology can be further extended for the processing of honeybee pupae protein in industrial production.

关 键 词：蜂蛹 双酶复合水解 响应面优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]